Relative Tyrosine Phosphorylation Index of the 77- to 89-kD Doublet and the 64-kD Band at 30 Seconds and 240 Seconds Aggregation Time Points in Controls and Patients Platelets
| Scanned Bands . | Fold Enhancement at 30 Seconds . | Fold Enhancement at 240 Seconds . | ||
|---|---|---|---|---|
| . | 77-80 kD . | 64 kD . | 77-80 kD . | 64 kD . |
| Controls | 5 ± 0.3 | 1.5 ± 0.3 | 3.9 ± 0.5 | 18.6 ± 2.1 |
| Patient B (type I) | 4.6-6.0 | 0.5-0.9 | 0.7-0.9 | 8-10 |
| Patient H (type II) | 4.5-5.5 | 1.3-1.5 | 0.6-1.0 | ND |
| Patient P (variant) | 2.3-3.7 | 0.7-1.3 | 1.1-1.7 | 9.7-12.1 |
| Scanned Bands . | Fold Enhancement at 30 Seconds . | Fold Enhancement at 240 Seconds . | ||
|---|---|---|---|---|
| . | 77-80 kD . | 64 kD . | 77-80 kD . | 64 kD . |
| Controls | 5 ± 0.3 | 1.5 ± 0.3 | 3.9 ± 0.5 | 18.6 ± 2.1 |
| Patient B (type I) | 4.6-6.0 | 0.5-0.9 | 0.7-0.9 | 8-10 |
| Patient H (type II) | 4.5-5.5 | 1.3-1.5 | 0.6-1.0 | ND |
| Patient P (variant) | 2.3-3.7 | 0.7-1.3 | 1.1-1.7 | 9.7-12.1 |
The intensity of the 77-80–kD and the 64-kD bands were measured by scanning densitometry after 30 seconds and 240 seconds thrombin activation time points. Linearity of of the densitometric signals was checked for both bands by increasing exposure times. A relative tyrosine phosphorylation index was calculated for a given band in a given sample as the ratio of densitometric values obtained at 30 seconds or 240 seconds versus that obtained in absence of activation in the same sample. Values for controls were derived from 5 autoluminograms in 5 independent experiments and are expressed as mean ± standard error of mean. The two values indicated for patients were derived from 2 separate Western blots and 2 autoluminograms from the same experiment.
Abbreviation: ND, not determined.