Thrombin Generation by Apoptotic VSMCs and Platelets
| . | No. of Experiments . | AUC (nmol⋅min/L) . | Peak Thrombin (nmol/L) . |
|---|---|---|---|
| Buffer C | 8 | 43.8 ± 3.4* | 26.3 ± 2.5* |
| Platelets | 14 | 220.8 ± 14.8* | 98.6 ± 7.4* |
| Activated platelets | 15 | 331.5 ± 15.1† | 172.5 ± 7.8† |
| Apoptotic VSMC-myc cells (2.24 × 109/L) | 8 | 304.6 ± 16.9 | 154.5 ± 9.0 |
| Apoptotic VSMC-myc cells (1.32 × 109/L) | 2 | 267.0 ± 5.4 | 130.9 ± 11.1 |
| Apoptotic VSMC-myc cells (0.73 × 109/L) | 2 | 170.0 ± 3.2 | 75.8 ± 1.0 |
| Apoptotic VSMC-myc cells (0.11 × 109/L) | 2 | 90.4 ± 0.4 | 39.2 ± 0.7 |
| Apoptotic human E1A cells | 2 | 211.0 ± 8.5‡ | 102.8 ± 4.1‡ |
| . | No. of Experiments . | AUC (nmol⋅min/L) . | Peak Thrombin (nmol/L) . |
|---|---|---|---|
| Buffer C | 8 | 43.8 ± 3.4* | 26.3 ± 2.5* |
| Platelets | 14 | 220.8 ± 14.8* | 98.6 ± 7.4* |
| Activated platelets | 15 | 331.5 ± 15.1† | 172.5 ± 7.8† |
| Apoptotic VSMC-myc cells (2.24 × 109/L) | 8 | 304.6 ± 16.9 | 154.5 ± 9.0 |
| Apoptotic VSMC-myc cells (1.32 × 109/L) | 2 | 267.0 ± 5.4 | 130.9 ± 11.1 |
| Apoptotic VSMC-myc cells (0.73 × 109/L) | 2 | 170.0 ± 3.2 | 75.8 ± 1.0 |
| Apoptotic VSMC-myc cells (0.11 × 109/L) | 2 | 90.4 ± 0.4 | 39.2 ± 0.7 |
| Apoptotic human E1A cells | 2 | 211.0 ± 8.5‡ | 102.8 ± 4.1‡ |
Apoptotis was induced by serum starvation of rat VSMCs expressing c-myc or normal human coronary VSMCs expressing the adenovirus gene E1A. Apoptotic cells were suspended to give an assay concentration of 2.24 × 109/L (except where otherwise stated), giving approximately the same surface area as 50 × 109/L unactivated or calcium-ionophore–activated platelets. Defibrinated plasma was activated with Russel's Viper Venom, and the thrombin generated after the addition of platelets or apoptotic VSMCs was measured in a chromogenic assay. The table shows the area under the thrombin generation curves (AUC) and the peak thrombin concentration. Values are mean ± SEM.
P < .005 compared with apoptotic VSMC-myc cells.
P = not significant compared with apoptotic VSMC-myc cells.
P < .0001 compared with buffer C.