Table 4. Neutrophil Adhesion to Fibrin:... | American Society of Hematology

Table 4.

Neutrophil Adhesion to Fibrin: Effect of MoAbs

Condition	Fibrin PMNs	80 mPa		200 mPa
		Total Cells per mm²	%	Total Cells per mm²	%
Surface: Fb-flow
Control		943 ± 98	100	287 ± 31	100
CD16	X	1,070 ± 137	115	ND
CD16	X	970 ± 80	105	283 ± 56	96
CD18	X	119 ± 32	12^4-150	13 ± 6	4^4-150
CD11a	X	1,018 ± 125	108	277 ± 43	99
CD11b	X	147 ± 35	15^4-150	39 ± 18	12^4-150
CD18 + CD11b	X	65 ± 20	8^4-150	ND
P-selectin	X	820 ± 174	85	315 ± 45	111
Neuraminidase	X	913 ± 137	95	ND

Surface: Fb-static
Control		1,168 ± 199	100	252 ± 40	100
CD16	X	1,334 ± 220	120	ND
CD18	X	177 ± 71	14^4-150	8 ± 1	4^4-150
CD11b	X	166 ± 54	13^4-150	12 ± 3	6^4-150

Condition	Fibrin PMNs	80 mPa		200 mPa
		Total Cells per mm²	%	Total Cells per mm²	%
Surface: Fb-flow
Control		943 ± 98	100	287 ± 31	100
CD16	X	1,070 ± 137	115	ND
CD16	X	970 ± 80	105	283 ± 56	96
CD18	X	119 ± 32	12^4-150	13 ± 6	4^4-150
CD11a	X	1,018 ± 125	108	277 ± 43	99
CD11b	X	147 ± 35	15^4-150	39 ± 18	12^4-150
CD18 + CD11b	X	65 ± 20	8^4-150	ND
P-selectin	X	820 ± 174	85	315 ± 45	111
Neuraminidase	X	913 ± 137	95	ND

Surface: Fb-static
Control		1,168 ± 199	100	252 ± 40	100
CD16	X	1,334 ± 220	120	ND
CD18	X	177 ± 71	14^4-150	8 ± 1	4^4-150
CD11b	X	166 ± 54	13^4-150	12 ± 3	6^4-150

Isolated neutrophils were perfused over cover slips covered with fibrin formed from plasma under flow conditions (Fb-flow) or static conditions (Fb-static). Neutrophils or fibrin were incubated with antibodies or neuraminidase as indicated. Cover slips with Fb-flow were incubated with control HEPES buffer, CD16 antibodies (CLB-FcR gran/1), or anti–P-selectin (Waps 12.2). The Fb-flow surface was also incubated with neuraminidase (0.25 U/mL for 15 minutes at 37°C). Neutrophils were incubated with control HEPES buffer, CD16 antibodies, CD18 antibodies (IB4), CD11a antibodies (TS-1), or CD11b antibodies (44a) for 30 minutes at room temperature. Then, the neutrophils (2 × 10⁶/mL HEPES buffer) were perfused in the presence of the MoAbs at 37°C at shear stresses of 80 or 200 mPa. Data represent the total number of adhering and rolling neutrophils per mm² and the percentage of the buffer control (mean ± SE, n = 4 to 10).

Abbreviation: ND, not determined.

F4-150

Statistically significant effect of antibody treatment compared with control cells was determined by ANOVA (P < .05).

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