Neutrophil Adhesion to Fibrin Formed Under Flow and Static Conditions (total cells/mm2)
| Surface . | 80 mPa . | 200 mPa . | 320 mPa . |
|---|---|---|---|
| Fb-flow | 1,031 ± 113 | 352 ± 32 | 101 ± 23 |
| Fb-static | 1,153 ± 126 (101%) | 163 ± 333-150 (46%) | 21 ± 153-150 (16%) |
| Surface . | 80 mPa . | 200 mPa . | 320 mPa . |
|---|---|---|---|
| Fb-flow | 1,031 ± 113 | 352 ± 32 | 101 ± 23 |
| Fb-static | 1,153 ± 126 (101%) | 163 ± 333-150 (46%) | 21 ± 153-150 (16%) |
Cover slips coated with tissue factor–rich matrix of HUVECs were either incubated with heparinized PPP (20 minutes at 37°C), forming Fb-static, or perfused with heparinized PPP at shear stress 20 mPa (20 minutes at 37°C), forming Fb-flow. Subsequently, the surfaces were rinsed and used for PMN perfusion at different shear stresses. Data represent the total number of adhering and rolling neutrophils per mm2 (mean ± SE, n = 4 to 10) and in parentheses the percentage of cells compared with Fb-flow at the same shear stress.
PMN adhesion to Fb-static was decreased significantly compared with Fb-flow as determined by paired Student's t-test (P < .01).