PMN Adhesion to Fibrin(ogen) and Platelet Surfaces (total cells/mm2)
| Surface . | 80 mPa . | 200 mPa . |
|---|---|---|
| Fb-flow | 1,031 ± 99 | 315 ± 30 |
| Fb-static | 1,100 ± 40 | 161 ± 32* |
| Platelets | 1,300 ± 120 | 803 ± 124* |
| Fb + platelets | 1,251 ± 79 | 887 ± 157* |
| Fb-flow (no platelets) | 1,004 ± 111 | 263 ± 42 |
| Fibrinogen-pure | 1,049 ± 85 | 200 ± 54 |
| Fb-pure | 946 ± 107 | 213 ± 95 |
| ECM-control | 200 ± 40* | 19 ± 11* |
| Surface . | 80 mPa . | 200 mPa . |
|---|---|---|
| Fb-flow | 1,031 ± 99 | 315 ± 30 |
| Fb-static | 1,100 ± 40 | 161 ± 32* |
| Platelets | 1,300 ± 120 | 803 ± 124* |
| Fb + platelets | 1,251 ± 79 | 887 ± 157* |
| Fb-flow (no platelets) | 1,004 ± 111 | 263 ± 42 |
| Fibrinogen-pure | 1,049 ± 85 | 200 ± 54 |
| Fb-pure | 946 ± 107 | 213 ± 95 |
| ECM-control | 200 ± 40* | 19 ± 11* |
Glass cover slips coated with tissue factor–rich ECM of PMA-stimulated HUVECs were treated under different conditions. This pretreatment resulted in cover slip coating with plasma factors and platelets in different combinations and quantities (see Table 1). Subsequently, the surfaces were used in a PMN perfusion. Isolated neutrophils were perfused at a shear stress of 80 or 200 mPa, and the number of adhered PMNs was measured after 5 minutes. Data represent the total number of adhering and rolling neutrophils per mm2 (mean ± SE, n = 4 to 10).
Statistically significant difference compared with Fb-flow was determined by ANOVA (P < .05).