Characteristics of Fibrin(ogen) and Platelet Surfaces
| Surface . | Procedure . | Fibrin/Fibrinogen . | Platelet Coverage (%) . | Plasma Proteins . |
|---|---|---|---|---|
| Fb-flow | PPP perfused over ECM (at shear stress 20 mPa) | Fibrin | <1 | + |
| Fb-static | ECM incubated with PPP | Fibrin | <1 | + |
| Platelets | Citrated whole blood perfused over ECM | — | 85 | + |
| Fb + platelets | Citrated whole blood perfused over a Fb-flow surface | Fibrin | 55 | + |
| Fb-flow (no platelets) | Filtered plasma perfused over ECM | Fibrin | 0 | + |
| Fibrinogen-pure | Fibrinogen coated on a glass cover slip | Fibrinogen | 0 | − |
| Fb-pure | Fibrinogen-pure incubated with thrombin | Fibrin | 0 | − |
| ECM-control | Tissue factor–rich ECM on a glass cover slip | — | 0 | + |
| Surface . | Procedure . | Fibrin/Fibrinogen . | Platelet Coverage (%) . | Plasma Proteins . |
|---|---|---|---|---|
| Fb-flow | PPP perfused over ECM (at shear stress 20 mPa) | Fibrin | <1 | + |
| Fb-static | ECM incubated with PPP | Fibrin | <1 | + |
| Platelets | Citrated whole blood perfused over ECM | — | 85 | + |
| Fb + platelets | Citrated whole blood perfused over a Fb-flow surface | Fibrin | 55 | + |
| Fb-flow (no platelets) | Filtered plasma perfused over ECM | Fibrin | 0 | + |
| Fibrinogen-pure | Fibrinogen coated on a glass cover slip | Fibrinogen | 0 | − |
| Fb-pure | Fibrinogen-pure incubated with thrombin | Fibrin | 0 | − |
| ECM-control | Tissue factor–rich ECM on a glass cover slip | — | 0 | + |
Glass cover slips coated with tissue factor–rich ECM of PMA-stimulated HUVECs were treated under different conditions. This pretreatment resulted in cover slip coating with fibrin, fibrinogen, platelets, and plasma factors in different combinations and quantities. The presence of fibrin(ogen) and the percentage of surface covered with platelets was evaluated by light microscopy and image analysis (see Fig 1).