Detection of Clones With Identical Ig/c-mycHybrid Sequences in Different Regions of the Intestine
| Strain . | Pristane3-150 . | Intestinal Distribution3-151 . | |||
|---|---|---|---|---|---|
| . | . | Duodenum . | Jejunum . | Ileum . | Colon . |
| BALB/cAn3-152 | − | − | x − | − − − − − x | − |
| BALB/cAn | − | − | − − | − − − − x x | − |
| BALB/cAn | + | x | − − | x − x − − − | − |
| BALB/cAn | + | − | − − | − − − x − x | − |
| BALB/cAn | + | x | − − | − − − x x − | − |
| C3H/HeJ | + | − | − − | − − − x x x | − |
| C3H/HeJ | + | − | − x | x − − − − − | − |
| C57BL/6 | + | x | − − | − − − − x − | − |
| Strain . | Pristane3-150 . | Intestinal Distribution3-151 . | |||
|---|---|---|---|---|---|
| . | . | Duodenum . | Jejunum . | Ileum . | Colon . |
| BALB/cAn3-152 | − | − | x − | − − − − − x | − |
| BALB/cAn | − | − | − − | − − − − x x | − |
| BALB/cAn | + | x | − − | x − x − − − | − |
| BALB/cAn | + | − | − − | − − − x − x | − |
| BALB/cAn | + | x | − − | − − − x x − | − |
| C3H/HeJ | + | − | − − | − − − x x x | − |
| C3H/HeJ | + | − | − x | x − − − − − | − |
| C57BL/6 | + | x | − − | − − − − x − | − |
The PCR amplification of such a recombination is marked by a cross. Tissues that were negative for that particular clone could harbor cells with an unrelated Ig/c-myc sequence. Note that identical Ig/c-myc sequences were amplified from noncontiguous intestinal samples in several cases. Therefore, a cross-contamination during the dissection appears to be unlikely.
Before (−) or 7 days after (+) the injection of pristane.
DNA preparations were made from the entire duodenum, two pieces of the jejunum, six pieces of the ileum, and from the entire colon.
Each line represents a single clone of cells with identical Ig/c-myc sequences that was detected in different intestinal DNA preparations.