Frequency of Ig/c-myc Recombinations in Lymphoid Tissues of Inbred Strains of Mice
| Strain . | Susceptible to Plasmacytoma Induction . | No. of Mice Carrying Ig/c-myc . | Tissue-Specific Distribution . | Switch Region* . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | PP . | Mes/OG . | MLN . | Sp . | Gut . | Sα . | recSα . | Sμ . | ?† . |
| Normal mice | |||||||||||
| BALB/cAn | + | 7/12‡ | 3/9 | 0/8 | 1/8 | 1/8 | 8/30ρ | 8 | 1 | 3 | 0 |
| C.D2-Idh1-Pep3 | + | 6/10 | 4/10 | 0/10 | 3/10 | 0/10 | ND | 2 | 0 | 5 | 0 |
| DBA/2N | − | 2/10 | 0/10 | 0/10 | 1/10 | 1/10 | 0/10 | 0 | 0 | 1 | 1 |
| Day 7 post pristane | |||||||||||
| BALB/cAn | + | 20/22 | 9/181-155 | 9/18 | 3/19 | 10/22 | 19/30 | 27 | 3 | 10 | 2 |
| C.D2-Igh1c | ? | 7/10 | 7/10 | 1/10 | 3/10 | 0/10 | ND | 6 | 3 | 2 | 0 |
| DBA/2N | − | 7/21 | 6/18 | 0/18 | 0/18 | 0/21 | 1/30 | 1 | 1 | 5 | 0 |
| C57BL/6 | − | 3/14 | 1/11 | 0/12 | 0/12 | 0/14 | 3/30 | 0 | 0 | 2 | 1 |
| C3H/HeJ | − | 5/11 | 3/8 | 1/8 | 1/8 | 0/11 | 7/30 | 6 | 1 | 1 | 0 |
| Strain . | Susceptible to Plasmacytoma Induction . | No. of Mice Carrying Ig/c-myc . | Tissue-Specific Distribution . | Switch Region* . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | PP . | Mes/OG . | MLN . | Sp . | Gut . | Sα . | recSα . | Sμ . | ?† . |
| Normal mice | |||||||||||
| BALB/cAn | + | 7/12‡ | 3/9 | 0/8 | 1/8 | 1/8 | 8/30ρ | 8 | 1 | 3 | 0 |
| C.D2-Idh1-Pep3 | + | 6/10 | 4/10 | 0/10 | 3/10 | 0/10 | ND | 2 | 0 | 5 | 0 |
| DBA/2N | − | 2/10 | 0/10 | 0/10 | 1/10 | 1/10 | 0/10 | 0 | 0 | 1 | 1 |
| Day 7 post pristane | |||||||||||
| BALB/cAn | + | 20/22 | 9/181-155 | 9/18 | 3/19 | 10/22 | 19/30 | 27 | 3 | 10 | 2 |
| C.D2-Igh1c | ? | 7/10 | 7/10 | 1/10 | 3/10 | 0/10 | ND | 6 | 3 | 2 | 0 |
| DBA/2N | − | 7/21 | 6/18 | 0/18 | 0/18 | 0/21 | 1/30 | 1 | 1 | 5 | 0 |
| C57BL/6 | − | 3/14 | 1/11 | 0/12 | 0/12 | 0/14 | 3/30 | 0 | 0 | 2 | 1 |
| C3H/HeJ | − | 5/11 | 3/8 | 1/8 | 1/8 | 0/11 | 7/30 | 6 | 1 | 1 | 0 |
Abbreviations: PP, Peyer's patches; Mes/OG, mesentery/mesenteric OG; MLN, mesenteric lymph node; Sp, spleen; ND, not done.
The Ig locus that is fused to c-myc is shown. The PCR technique used detects recombinations between c-myc and Sα or Sμ. Rec Sα: an additional rearrangement between Sα and another Ig switch region was amplified adjacent to the Ig/c-myc recombination. The difference between the number of positive tissues and the number of recombinations is caused by the amplification of identical Ig/c-myc products in different tissues and by the detection of more than a single clone in some cases.
In four cases, the sequence that was joined to c-myc could not be aligned to germline.
Numbers represent positive mice or tissues per total number tested.
ρ In these mice, the gut was dissected into 10 different pieces.
One c-myc recombination found in the Peyer's patches was detected in the omentum as well. DNA was prepared from 10 omenta. The study also included eight bone marrows and six fetal livers that were found to be negative.