Table 1. DNA Genotyping: Primer... | American Society of Hematology

Table 1.

DNA Genotyping: Primer Sequences, PCR Conditions, and Sizes of PCR Products

Gene	Primers	Sequence 5′→3′	Exon	Hybridization T°	Cycles No.	[MgCl₂] mmol/L	Size of PCR Product	Enzymes
IDS	IDS 3b	CCAAAGAAGG	Intron 3	48	35	1.5	151	HpaII
		GAGGGTCCAC
	(sense)
	IDS 4	AGACCAGCTAT	4
		ACGGAGAATCA
	(antisense)	C*C
P55	P55 DNAs	ATCTCAAATTC	Intron 2	44	35	2.0	192	Dde I
		CTCCTCAAA
	(sense)
	P55 DNAas	TGTTTTTCATTC	3
		AGCTTCT*G
	(antisense)
G6PD	G6PD-E10	GCTGGACCTGA	10	59	35	3.0	183	Pvu I
		CCTACGGCAAC
	(sense)	A
	G6PVPDPVU1	GAAGACGTCCA	11
		GGATGAGGCG
	(antisense)	A*TC

Gene	Primers	Sequence 5′→3′	Exon	Hybridization T°	Cycles No.	[MgCl₂] mmol/L	Size of PCR Product	Enzymes
IDS	IDS 3b	CCAAAGAAGG	Intron 3	48	35	1.5	151	HpaII
		GAGGGTCCAC
	(sense)
	IDS 4	AGACCAGCTAT	4
		ACGGAGAATCA
	(antisense)	C*C
P55	P55 DNAs	ATCTCAAATTC	Intron 2	44	35	2.0	192	Dde I
		CTCCTCAAA
	(sense)
	P55 DNAas	TGTTTTTCATTC	3
		AGCTTCT*G
	(antisense)
G6PD	G6PD-E10	GCTGGACCTGA	10	59	35	3.0	183	Pvu I
		CCTACGGCAAC
	(sense)	A
	G6PVPDPVU1	GAAGACGTCCA	11
		GGATGAGGCG
	(antisense)	A*TC

Indicate the mismatched nucleotides introduced by the primer to create restriction sites.