Affinity purification and MS results of ALAS2
| Murine protein . | |||||
|---|---|---|---|---|---|
| Human input . | Kdh E1 . | Kdh E2 . | Kdh E3 . | Alas2 . | Fumarate hydratase . |
| ALAS2 (n = 2) | 20-45 | 10-23 | 10-20 | 90-347 | 4-10 |
| 15-33 | 7-13 | 6-10 | 22-34 | 4-8 | |
| 17-29% | 19-25% | 16-19% | 42-56% | 11-13% | |
| Empty vector (n = 2) | 0-2 | 6-7 | 0-4 | 0 | 3-6 |
| 0-2 | 2-3 | 0-3 | 0 | 2-3 | |
| 0-2% | 7-10% | 0-7% | 0% | 6-7% | |
| Murine protein . | |||||
|---|---|---|---|---|---|
| Human input . | Kdh E1 . | Kdh E2 . | Kdh E3 . | Alas2 . | Fumarate hydratase . |
| ALAS2 (n = 2) | 20-45 | 10-23 | 10-20 | 90-347 | 4-10 |
| 15-33 | 7-13 | 6-10 | 22-34 | 4-8 | |
| 17-29% | 19-25% | 16-19% | 42-56% | 11-13% | |
| Empty vector (n = 2) | 0-2 | 6-7 | 0-4 | 0 | 3-6 |
| 0-2 | 2-3 | 0-3 | 0 | 2-3 | |
| 0-2% | 7-10% | 0-7% | 0% | 6-7% | |
Data shown top to bottom as spectral counts, number of unique peptides, and percent coverage, respectively.58