IC50 values of the Syk inhibitors SYK II, SYK IV, and R406 in 2 different P falciparum laboratory strains (Palo Alto and Dd2) and ex vivo grown isolates from Vietnamese malaria patients
| . | 24 h . | 48 h . |
|---|---|---|
| Palo Alto strain | ||
| Syk inhibitors | IC50 (μΜ) | IC50 (μΜ) |
| SYK II | 5.01 ± 0.44 | 0.9 ± 0.16 |
| SYK IV | 7.28 ± 0.4 | 1.75 ± 0.31 |
| R406 | 2.62 ± 0.83 | 0.55 ± 0.19 |
| Dd2 strain | ||
| Syk inhibitors | IC50 (μΜ) | IC50 (μΜ) |
| SYK II | 4.62 ± 0.44 | 0.68 ± 0.15 |
| SYK IV | 6.39 ± 0.46 | 1.28 ± 0.28 |
| R406 | 3.58 ± 0.37 | 0.69 ± 0.26 |
| Ex vivo–grown isolates from Vietnam | ||
| SYK II (N = 23) | 4.48 ± 0.37 | 0.71 ± 0.9 |
| R406 (N= 23) | 3.02 ± 0.23 | 0.44 ± 0.4 |
| . | 24 h . | 48 h . |
|---|---|---|
| Palo Alto strain | ||
| Syk inhibitors | IC50 (μΜ) | IC50 (μΜ) |
| SYK II | 5.01 ± 0.44 | 0.9 ± 0.16 |
| SYK IV | 7.28 ± 0.4 | 1.75 ± 0.31 |
| R406 | 2.62 ± 0.83 | 0.55 ± 0.19 |
| Dd2 strain | ||
| Syk inhibitors | IC50 (μΜ) | IC50 (μΜ) |
| SYK II | 4.62 ± 0.44 | 0.68 ± 0.15 |
| SYK IV | 6.39 ± 0.46 | 1.28 ± 0.28 |
| R406 | 3.58 ± 0.37 | 0.69 ± 0.26 |
| Ex vivo–grown isolates from Vietnam | ||
| SYK II (N = 23) | 4.48 ± 0.37 | 0.71 ± 0.9 |
| R406 (N= 23) | 3.02 ± 0.23 | 0.44 ± 0.4 |
Syk inhibitors were added to synchronized parasite cultures at 16 to 20 hours postinvasion. The IC50 was determined after 24 and 48 hours incubation of the treated cultures by counting the parasitemia during the 2 subsequent erythrocytic cycles (40-44 and 64-68 hours after first invasion).