Table 4.

NOTCH-associated pathway gene expression as assessed in the RNA-Seq analysis of cGVHD B cells after NOTCH2 blockade

Gene↑ or ↓log2 fold changePAdjusted PRank (of 12 880)
HES4 ↓ −2.97 2.8 × 10−17 1.8 × 10−13 2 
HES1 ↓ −1.10 3.8 × 10−4 .062 80 
LFNG ↑ 0.660 .001 .105 133 
HDAC2 ↓ −0.541 .014 .321 543 
MFNG ↓ −0.525 .016 .339 622 
EP300 — 0.667 .061 .454 1 737 
MAML2 — 0.552 .119 .510 2 997 
NOTCH2 — 0.371 .165 .550 3 867 
DTX2 — 0.300 .262 .622 5 430 
HDAC1 — −0.101 .595 .828 9 259 
DTX1 — 0.030 .926 .976 12 209 
DTX4 — 0.026 .933 .979 12 269 
Gene↑ or ↓log2 fold changePAdjusted PRank (of 12 880)
HES4 ↓ −2.97 2.8 × 10−17 1.8 × 10−13 2 
HES1 ↓ −1.10 3.8 × 10−4 .062 80 
LFNG ↑ 0.660 .001 .105 133 
HDAC2 ↓ −0.541 .014 .321 543 
MFNG ↓ −0.525 .016 .339 622 
EP300 — 0.667 .061 .454 1 737 
MAML2 — 0.552 .119 .510 2 997 
NOTCH2 — 0.371 .165 .550 3 867 
DTX2 — 0.300 .262 .622 5 430 
HDAC1 — −0.101 .595 .828 9 259 
DTX1 — 0.030 .926 .976 12 209 
DTX4 — 0.026 .933 .979 12 269 

cGVHD B cells after NOTCH2 blockade from the experiment described in Table 2. P values and adjusted P values are indicated. The numerical rank for each gene of all 12 880 genes expressed above background is also indicated, based on the adjusted P value score.

—, Not significantly changed by NOTCH2 blockade.

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