Comparison of ROS generation in cardiomyocyte (H9C2) and a hepatocyte (HuH7) cell lines is shown after addition of clinically available chelators or ELT, alone or in combination over the course of 90 minutes
| . | H9C2 cardiomyocyte-like cells . | HuH7 hepatocyte-like cells . |
|---|---|---|
| DFO 1 μM IBE | 12.1 ± 1.3 | 14.3 ± 0.9 |
| DFP 1 μM IBE | 29.7 ± 1.3 | 25.0 ± 1.8 |
| DFX 1 μM IBE | 33.2 ± 2.7 | 21.4 ± 2.6 |
| ELT 1 μM | 42.2 ± 1.7 | 39.2 ± 2.1 |
| ELT 1 μM + DFO 1 μM IBE | 62.2 ± 3.2* | 55.1 ± 3.1** |
| ELT 1 μM + DFP 1 μM IBE | 60.3 ± 4.1* | 60.2 ± 3.2* |
| ELT 1 μM + DFX 1 μM IBE | 57.3 ± 3.3** | 51.3 ± 2.2* |
| . | H9C2 cardiomyocyte-like cells . | HuH7 hepatocyte-like cells . |
|---|---|---|
| DFO 1 μM IBE | 12.1 ± 1.3 | 14.3 ± 0.9 |
| DFP 1 μM IBE | 29.7 ± 1.3 | 25.0 ± 1.8 |
| DFX 1 μM IBE | 33.2 ± 2.7 | 21.4 ± 2.6 |
| ELT 1 μM | 42.2 ± 1.7 | 39.2 ± 2.1 |
| ELT 1 μM + DFO 1 μM IBE | 62.2 ± 3.2* | 55.1 ± 3.1** |
| ELT 1 μM + DFP 1 μM IBE | 60.3 ± 4.1* | 60.2 ± 3.2* |
| ELT 1 μM + DFX 1 μM IBE | 57.3 ± 3.3** | 51.3 ± 2.2* |
Experimental design and determination of ROS is otherwise as described in Figure 3. Results are the mean ± SEM of 4 observations in 1 experiment. (*P < 0.05, **P < 0.01 compared with control) percentage decrease in ROS generation rate at 90 minutes.