Comparison of percentage of intracellular iron mobilized at 8 hours in a cardiomyocyte (H9C2) and a hepatocyte (HuH7) cell lines by clinically available chelators or ELT, alone or in combination
| . | H9C2 cardiomyocyte-like cells . | HuH7 hepatocyte-like cells . |
|---|---|---|
| DFO 1 μM IBE | 14.2 ± 1.2 | 28.6 ± 1.4 |
| DFP 1 μM IBE | 17.1 ± 1.5 | 25.7 ± 1.2 |
| DFX 1 μM IBE | 11.7 ± 1.0 | 22.8 ± 1.7 |
| ELT 1 μM | 42.1 ± 2.1 | 6.9 ± 0.3 |
| ELT 1 μM + DFO 1 μM IBE | 58.2 ± 2.5 | 34.3 ± 1.1 |
| ELT 1 μM + DFP 1 μM IBE | 59.9 ± 3.1 | 38.2 ± 1.5 |
| ELT 1 μM + DFX 1 μM IBE | 66.4 ± 2.4 | 51.4 ± 1.9 |
| . | H9C2 cardiomyocyte-like cells . | HuH7 hepatocyte-like cells . |
|---|---|---|
| DFO 1 μM IBE | 14.2 ± 1.2 | 28.6 ± 1.4 |
| DFP 1 μM IBE | 17.1 ± 1.5 | 25.7 ± 1.2 |
| DFX 1 μM IBE | 11.7 ± 1.0 | 22.8 ± 1.7 |
| ELT 1 μM | 42.1 ± 2.1 | 6.9 ± 0.3 |
| ELT 1 μM + DFO 1 μM IBE | 58.2 ± 2.5 | 34.3 ± 1.1 |
| ELT 1 μM + DFP 1 μM IBE | 59.9 ± 3.1 | 38.2 ± 1.5 |
| ELT 1 μM + DFX 1 μM IBE | 66.4 ± 2.4 | 51.4 ± 1.9 |
Chelator concentrations are expressed as IBE. Cells were preloaded with iron, rinsed, and incubated with chelators, and cellular iron was determined as described in Figure 2. Results shown as the percentage iron released expressed as a percentage of T0 cellular iron and are the mean ± SEM of 4 replicates in 1 experiment percentage iron release after 8 hours of chelator treatment.