Chimerism and immunological reconstitution in 21 survivors after HSCT
| . | . | Chimerism . | Immunological reconstitution . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Patient No. . | Follow-up, y . | Full blood, % donor . | T cells, % donor . | Other leukocyte subpopulations, % donor . | Neutrophils/µL . | T cells/µL . | B cells/µL . | NK cells/µL . | Naïve T cells, % . | IVIG . | Specific antibodies . |
| 2 | 23.6 | mixed | 100 | Non–T cells: >90, PMN: >95; CD34+: <10; red blood cells: 6 | 1148 | 1270 | 109 | 109 | 34 | No | Tet/Diph/HiB/Pneu |
| 3 | 7.9 | n.d. | 100 | B cells: 100 | 4100 | n.d. | n.d. | n.d. | n.d. | No | n.d. |
| 4 | 3.0 | 100 | n.d. | CD34+: 100 | 1760 | 5350 | 1110 | 490 | 58 | No | Tet/Diph/HiB/MMR |
| 5 | 8.0 | n.d. | 100 | B cells: 90, PMN: 90 | 2410 | 1553 | 263 | 174 | 66 | No | Tet/Diph/HiB/Pneu/MMR |
| 6 | 2.7 | 100 | 100 | PMN: 100 | 1600 | 3620 | 860 | 240 | 54 | No | Tet/Diph/HiB/Pneu |
| 7 | 2.1 | 87 | n.d. | n.d. | 400 | 1138 | 121 | 67 | n.d. | No | n.d. |
| 8 | 11.0 | 100 | 100 | B cells: 100, PMN: 100 | 1780 | 2487 | 324 | 367 | 68 | No | Tet/Diph/HiB/Pneu/MMR |
| 9 | 4.1 | 100 | n.d. | n.d. | 1530 | 1060 | 620 | 70 | 70 | No | Tet/Diph/HiB/Pneu |
| 10 | 7.4 | 100 | 100 | PMN: 99 | 2260 | 3290 | 870 | 786 | 52 | No | Tet/Diph/HiB/Pneu/MMR |
| 11 | 6.5 | 100 | n.d. | n.d. | 2400 | 3000 | 1300 | 807 | n.d. | No | Tet/Diph/HiB/Pneu/MMR |
| 12 | 5.0 | 100 | 100 | B cells: 100; NK cells: 100, PMN: 100 | 1776 | 1520 | 830 | 200 | n.d. | No | MMR |
| 13 | 3.1 | 100 | n.d. | n.d. | 4563 | 2084 | 322 | 275 | 66 | Yes | unknown |
| 14 | 3.4 | n.d. | 96 | B cells: 33, PMN: 75 | 2170 | 1262 | 177 | 397 | n.d. | Yes | n.d. |
| 18 | 1.1 | 100 | n.d. | n.d. | 3000 | 1580 | 200 | 360 | n.d. | Unknown | n.d. |
| 21 | 16.6 | 100 | n.d. | n.d. | 3700 | n.d. | n.d. | n.d. | n.d. | No | Tet/Diph |
| 22 | 4.4 | 100 | 100 | n.d. | 2110 | 3410 | 810 | 270 | 51 | No | Tet/Diph/HiB/Pneu |
| 23 | 17.1 | 100 | 100 | red blood cells: 14 | 2960 | 1930 | 210 | 115 | 39 | No | Tet/Diph/HiB/Pneu |
| 27 | 17.0 | n.d. | 100 | B cells: 95, PMN: 100 | 900 | 461 | 77 | 13 | 65 | No | Tet/Diph/HiB/Pneu/MMR |
| 28 | 12.9 | mixed | 100 | CD34+: 80, non–T cells: >95, PMN: 100; red blood cells: 50 | 1672 | 1315 | 307 | 68 | 49 | No | Tet/Diph/HiB/Pneu |
| 31 | 0.6 | 100 | n.d. | n.d. | 3946 | 480 | 70 | 50 | 0 | Yes | n.d. |
| 32 | 9.1 | 100 | n.d. | n.d. | 3400 | 2870 | 350 | 140 | n.d. | No | VZV |
| . | . | Chimerism . | Immunological reconstitution . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Patient No. . | Follow-up, y . | Full blood, % donor . | T cells, % donor . | Other leukocyte subpopulations, % donor . | Neutrophils/µL . | T cells/µL . | B cells/µL . | NK cells/µL . | Naïve T cells, % . | IVIG . | Specific antibodies . |
| 2 | 23.6 | mixed | 100 | Non–T cells: >90, PMN: >95; CD34+: <10; red blood cells: 6 | 1148 | 1270 | 109 | 109 | 34 | No | Tet/Diph/HiB/Pneu |
| 3 | 7.9 | n.d. | 100 | B cells: 100 | 4100 | n.d. | n.d. | n.d. | n.d. | No | n.d. |
| 4 | 3.0 | 100 | n.d. | CD34+: 100 | 1760 | 5350 | 1110 | 490 | 58 | No | Tet/Diph/HiB/MMR |
| 5 | 8.0 | n.d. | 100 | B cells: 90, PMN: 90 | 2410 | 1553 | 263 | 174 | 66 | No | Tet/Diph/HiB/Pneu/MMR |
| 6 | 2.7 | 100 | 100 | PMN: 100 | 1600 | 3620 | 860 | 240 | 54 | No | Tet/Diph/HiB/Pneu |
| 7 | 2.1 | 87 | n.d. | n.d. | 400 | 1138 | 121 | 67 | n.d. | No | n.d. |
| 8 | 11.0 | 100 | 100 | B cells: 100, PMN: 100 | 1780 | 2487 | 324 | 367 | 68 | No | Tet/Diph/HiB/Pneu/MMR |
| 9 | 4.1 | 100 | n.d. | n.d. | 1530 | 1060 | 620 | 70 | 70 | No | Tet/Diph/HiB/Pneu |
| 10 | 7.4 | 100 | 100 | PMN: 99 | 2260 | 3290 | 870 | 786 | 52 | No | Tet/Diph/HiB/Pneu/MMR |
| 11 | 6.5 | 100 | n.d. | n.d. | 2400 | 3000 | 1300 | 807 | n.d. | No | Tet/Diph/HiB/Pneu/MMR |
| 12 | 5.0 | 100 | 100 | B cells: 100; NK cells: 100, PMN: 100 | 1776 | 1520 | 830 | 200 | n.d. | No | MMR |
| 13 | 3.1 | 100 | n.d. | n.d. | 4563 | 2084 | 322 | 275 | 66 | Yes | unknown |
| 14 | 3.4 | n.d. | 96 | B cells: 33, PMN: 75 | 2170 | 1262 | 177 | 397 | n.d. | Yes | n.d. |
| 18 | 1.1 | 100 | n.d. | n.d. | 3000 | 1580 | 200 | 360 | n.d. | Unknown | n.d. |
| 21 | 16.6 | 100 | n.d. | n.d. | 3700 | n.d. | n.d. | n.d. | n.d. | No | Tet/Diph |
| 22 | 4.4 | 100 | 100 | n.d. | 2110 | 3410 | 810 | 270 | 51 | No | Tet/Diph/HiB/Pneu |
| 23 | 17.1 | 100 | 100 | red blood cells: 14 | 2960 | 1930 | 210 | 115 | 39 | No | Tet/Diph/HiB/Pneu |
| 27 | 17.0 | n.d. | 100 | B cells: 95, PMN: 100 | 900 | 461 | 77 | 13 | 65 | No | Tet/Diph/HiB/Pneu/MMR |
| 28 | 12.9 | mixed | 100 | CD34+: 80, non–T cells: >95, PMN: 100; red blood cells: 50 | 1672 | 1315 | 307 | 68 | 49 | No | Tet/Diph/HiB/Pneu |
| 31 | 0.6 | 100 | n.d. | n.d. | 3946 | 480 | 70 | 50 | 0 | Yes | n.d. |
| 32 | 9.1 | 100 | n.d. | n.d. | 3400 | 2870 | 350 | 140 | n.d. | No | VZV |
HiB, Haemophilus influenzae type B; Diph, diphtheria; IVIG: IV substitution of immunoglobulins; MMR: measles, mumps, and rubella; n.d., not determined; PMN, polymorphonuclear cell; Pneu, S pneumoniae; Tet, tetanus; VZV, varicella zoster virus.