Thrombin generation in reconstituted human whole blood
| . | No platelets, % . | 200 × 106/mL platelets, % . | 450 × 106/mL platelets, % . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| . | 0 . | 20 . | 45 . | 0 . | 20 . | 45 . | 0 . | 20 . | 45 . |
| Hematocrit | |||||||||
| Lag time, min | 9.1 ± 5.2 | 9.8 ± 2.0 | 12.9 ± 1.7 | 7.2 ± 1.0 | 7.3 ± 1.2 | 7.4 ± 0.6 | 7.4 ± 1.0 | 6.9 ± 0.8 | 7.2 ± 1.3 |
| Time to peak, min | 23.8 ± 10.0 | 14.2 ± 2.0 | 18.7 ± 2.7 | 13.2 ± 1.7 | 11.2 ± 1.6 | 11.2 ± 1.0 | 12.5 ± 1.4 | 10.9 ± 1.2 | 11.0 ± 1.7 |
| Rate, nM/min | 0.2 ± 0.1 | 4.7 ± 0.5* | 6.4 ± 1.1* | 3.0 ± 0.2 | 5.4 ± 1.0* | 8.5 ± 1.7* | 3.7 ± 1.9 | 6.1 ± 0.4 | 6.5 ± 1.1 |
| Peak, nM | 3.9 ± 2.0 | 51.8 ± 7.3* | 74.3 ± 23.0* | 42.9 ± 2.9 | 63.2 ± 17.1* | 84.0 ± 30.0* | 65.8 ± 34.9 | 64.1 ± 5.4 | 69.1 ± 15.9 |
| Endogenous thrombin potential, nM*min | 51.0 ± 50.5 | 382.3 ± 74.9* | 619.3 ± 209.9* | 418.8 ± 59.9 | 412.0 ± 149.5 | 525.2 ± 197.8 | 500.8 ± 261.1 | 414.0 ± 43.8 | 457.5 ± 122.2 |
| . | No platelets, % . | 200 × 106/mL platelets, % . | 450 × 106/mL platelets, % . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| . | 0 . | 20 . | 45 . | 0 . | 20 . | 45 . | 0 . | 20 . | 45 . |
| Hematocrit | |||||||||
| Lag time, min | 9.1 ± 5.2 | 9.8 ± 2.0 | 12.9 ± 1.7 | 7.2 ± 1.0 | 7.3 ± 1.2 | 7.4 ± 0.6 | 7.4 ± 1.0 | 6.9 ± 0.8 | 7.2 ± 1.3 |
| Time to peak, min | 23.8 ± 10.0 | 14.2 ± 2.0 | 18.7 ± 2.7 | 13.2 ± 1.7 | 11.2 ± 1.6 | 11.2 ± 1.0 | 12.5 ± 1.4 | 10.9 ± 1.2 | 11.0 ± 1.7 |
| Rate, nM/min | 0.2 ± 0.1 | 4.7 ± 0.5* | 6.4 ± 1.1* | 3.0 ± 0.2 | 5.4 ± 1.0* | 8.5 ± 1.7* | 3.7 ± 1.9 | 6.1 ± 0.4 | 6.5 ± 1.1 |
| Peak, nM | 3.9 ± 2.0 | 51.8 ± 7.3* | 74.3 ± 23.0* | 42.9 ± 2.9 | 63.2 ± 17.1* | 84.0 ± 30.0* | 65.8 ± 34.9 | 64.1 ± 5.4 | 69.1 ± 15.9 |
| Endogenous thrombin potential, nM*min | 51.0 ± 50.5 | 382.3 ± 74.9* | 619.3 ± 209.9* | 418.8 ± 59.9 | 412.0 ± 149.5 | 525.2 ± 197.8 | 500.8 ± 261.1 | 414.0 ± 43.8 | 457.5 ± 122.2 |
Thrombin generation was measured by whole blood–calibrated automated thrombography. Data show mean ± SD. N = 3-6 separate experiments per condition.
Analysis of variance with Dunnett’s post-hoc test, P < .05 vs 0% hematocrit for each platelet concentration.