Quantification of the number of α and δ granules in unstimulated and activated whole platelets
| . | No. of seconds of stimulation . | No. of platelets . | α granules per platelet . | δ granules per platelet . | ||
|---|---|---|---|---|---|---|
| No. . | P . | No. . | P . | |||
| Unstimulated platelets | 49 | 41.6 ± 4.3 | 8 ± 0.6 | |||
| ADP 5 µM | 7 | 8 | 40.6 ± 1.1 | 8.1 ± 0.3 | ||
| 30 | 13 | 38.7 ± 1.5 | 8.2 ± 0.6 | |||
| Thrombin 0.1 U/mL | 15 | 8 | 30.8 ± 1.4 | <.01 | 5.8 ± 0.5 | |
| 70 | 8 | 17.5 ± 1.2 | <.001 | 2.7 ± 0.6 | <.001 | |
| Thrombin 1 U/mL | 10 | 8 | 7.5 ± 0.9 | <.001 | 1.3 ± 0.3 | <.001 |
| 70 | 8 | 2.8 ± 0.8 | <.001 | None detected | ||
| . | No. of seconds of stimulation . | No. of platelets . | α granules per platelet . | δ granules per platelet . | ||
|---|---|---|---|---|---|---|
| No. . | P . | No. . | P . | |||
| Unstimulated platelets | 49 | 41.6 ± 4.3 | 8 ± 0.6 | |||
| ADP 5 µM | 7 | 8 | 40.6 ± 1.1 | 8.1 ± 0.3 | ||
| 30 | 13 | 38.7 ± 1.5 | 8.2 ± 0.6 | |||
| Thrombin 0.1 U/mL | 15 | 8 | 30.8 ± 1.4 | <.01 | 5.8 ± 0.5 | |
| 70 | 8 | 17.5 ± 1.2 | <.001 | 2.7 ± 0.6 | <.001 | |
| Thrombin 1 U/mL | 10 | 8 | 7.5 ± 0.9 | <.001 | 1.3 ± 0.3 | <.001 |
| 70 | 8 | 2.8 ± 0.8 | <.001 | None detected | ||
Values are presented with standard error of the mean. P values were determined by using the two-way analysis of variance to compare each number of α and δ granules to unstimulated platelets.