Binding affinities of anti-C1 mAbs for fVIII determined by SPR spectroscopy
| mAb . | Group . | ka (M-1s−1) . | kd (s−1) . | KD (nM) . | Rmax (RU) . | χ2 (RU2) . |
|---|---|---|---|---|---|---|
| 2A9 | A | (0.3 ± 0.1) × 106 | (2.2 ± 0.2) × 10−4 | 0.9 ± 0.2 | 97.8 ± 1.1 | 0.3 ± 0.2 |
| B153 | A | (0.3 ± 0.03) × 106 | (28 ± 10.8) × 10−4 | 10 ± 2.9 | 92.5 ± 22.8 | 1 ± 0.8 |
| F156 | A | (0.3 ± 0.1) × 106 | (8.3 ± 0.6) × 10−4 | 2.9 ± 1.5 | 74.0 ± 21.1 | 0.9 ± 1.2 |
| I41 | A | (0.3 ± 0.1) × 106 | (6.5 ± 0.4) × 10−4 | 1.9 ± 0.2 | 85.8 ± 3.3 | 0.1 ± 0.2 |
| I84 | A | (0.2 ± 0.03) × 106 | (3.5 ± 0.3) × 10−4 | 1.9 ± 0.5 | 108.9 ± 6.6 | 0.1 ± 0.04 |
| I88 | A | (0.4 ± 0.2) × 106 | (4.3 ± 1.1) × 10−4 | 1 ± 0.1 | 124.6 ± 11.2 | 0.2 ± 0.03 |
| M6143 | A | (0.5 ± 0.2) × 106 | (0.9 ± 0.1) × 10−4 | 0.2 ± 0.1 | 132.8 ± 25.3 | 0.1 ± 0.004 |
| B136 | B | (3.1 ± 1.2) × 106 | (3.2 ± 0.4) × 10−4 | 0.1 ± 0.03 | 301.9 ± 26.5 | 1.3 ± 0.04 |
| mAb . | Group . | ka (M-1s−1) . | kd (s−1) . | KD (nM) . | Rmax (RU) . | χ2 (RU2) . |
|---|---|---|---|---|---|---|
| 2A9 | A | (0.3 ± 0.1) × 106 | (2.2 ± 0.2) × 10−4 | 0.9 ± 0.2 | 97.8 ± 1.1 | 0.3 ± 0.2 |
| B153 | A | (0.3 ± 0.03) × 106 | (28 ± 10.8) × 10−4 | 10 ± 2.9 | 92.5 ± 22.8 | 1 ± 0.8 |
| F156 | A | (0.3 ± 0.1) × 106 | (8.3 ± 0.6) × 10−4 | 2.9 ± 1.5 | 74.0 ± 21.1 | 0.9 ± 1.2 |
| I41 | A | (0.3 ± 0.1) × 106 | (6.5 ± 0.4) × 10−4 | 1.9 ± 0.2 | 85.8 ± 3.3 | 0.1 ± 0.2 |
| I84 | A | (0.2 ± 0.03) × 106 | (3.5 ± 0.3) × 10−4 | 1.9 ± 0.5 | 108.9 ± 6.6 | 0.1 ± 0.04 |
| I88 | A | (0.4 ± 0.2) × 106 | (4.3 ± 1.1) × 10−4 | 1 ± 0.1 | 124.6 ± 11.2 | 0.2 ± 0.03 |
| M6143 | A | (0.5 ± 0.2) × 106 | (0.9 ± 0.1) × 10−4 | 0.2 ± 0.1 | 132.8 ± 25.3 | 0.1 ± 0.004 |
| B136 | B | (3.1 ± 1.2) × 106 | (3.2 ± 0.4) × 10−4 | 0.1 ± 0.03 | 301.9 ± 26.5 | 1.3 ± 0.04 |