TCRB gene sequence analysis for T-cell clones isolated from mild HA subjects GS1-17A and GS1-32A
| T-cell clone . | TCRBV gene* . | TCRBD gene* . | TCRBJ gene* . | Junction sequence* . | Sequence identifier† . |
|---|---|---|---|---|---|
| 17A-19WK-5‡ | 20-1§ | 1*01 | 1-2*01 | CSAHTRANYGYTF | Seq13 |
| 17A-19WK-10 | 20-1§ | 2*01 or *02 | 2-5*01 | CSAITGTSGETQYF | Seq14 |
| 17A-19WK-11 | 20-1§ | 2*01 or *02 | 2-7*01 | CSARGPSNGYEQYF | Seq15 |
| 17A-19WK-22 | 20-1§ | 2*01 or *02 | 2-7*01 | CSARGPSNGYEQYF | Seq15 |
| 17A-19WK-33 | 20-1§ | 2*01 or *02 | 2-7*01 | CSARGPSNGYEQYF | Seq15 |
| 17A-21MO-3 | 6-6*01 | 1*01 | 2-2*01 | CASSRQDTGELFF | Seq16 |
| 17A-21MO-4|| | 6-6*01 or *03 | Not identified | Not identified | Not identified | |
| 17A-21MO-5 | 6-6*01 or *03 | 1*01 | 2-2*01 | CASSRQDTGELFF | Seq16 |
| 17A-21MO-6 | 6-6*01 | 1*01 | 2-2*01 | CASSRQDTGELFF | Seq16 |
| 17A-21MO-7 | 6-6*01 | 1*01 | 2-2*01 | CASSRQDTGELFF | Seq16 |
| 17A-21MO-8¶ | 5-1*01 | 1*01 | 1-2*01 | CASSLDGRANYGYTF | Seq17 |
| 17A-21MO-11¶ | 5-1*01 | 1*01 | 1-2*01 | CASSLDGRANYGYTF | Seq17 |
| 17A-21MO-12¶ | 5-1*01 | 1*01 | 1-2*01 | CASSLDGRANYGYTF | Seq17 |
| 32A-5 | 6-1*01 | 1*01 | 2-7*01 | CASSDGQPIYEQYF | Seq18 |
| 32A-14 | 6-1*01 | 1*01 | 2-7*01 | CASSDGQPIYEQYF | Seq18 |
| 32A-15 | 6-1*01 | 1*01 | 2-7*01 | CASSDGQPIYEQYF | Seq18 |
| 32A-16 | 6-1*01 | 1*01 | 2-7*01 | CASSDGQPIYEQYF | Seq18 |
| 32A-18 | 6-1*01 | 1*01 | 2-7*01 | CASSDGQPIYEQYF | Seq18 |
| 32A-21 | 6-1*01 | 1*01 | 2-7*01 | CASSDGQPIYEQYF | Seq18 |
| T-cell clone . | TCRBV gene* . | TCRBD gene* . | TCRBJ gene* . | Junction sequence* . | Sequence identifier† . |
|---|---|---|---|---|---|
| 17A-19WK-5‡ | 20-1§ | 1*01 | 1-2*01 | CSAHTRANYGYTF | Seq13 |
| 17A-19WK-10 | 20-1§ | 2*01 or *02 | 2-5*01 | CSAITGTSGETQYF | Seq14 |
| 17A-19WK-11 | 20-1§ | 2*01 or *02 | 2-7*01 | CSARGPSNGYEQYF | Seq15 |
| 17A-19WK-22 | 20-1§ | 2*01 or *02 | 2-7*01 | CSARGPSNGYEQYF | Seq15 |
| 17A-19WK-33 | 20-1§ | 2*01 or *02 | 2-7*01 | CSARGPSNGYEQYF | Seq15 |
| 17A-21MO-3 | 6-6*01 | 1*01 | 2-2*01 | CASSRQDTGELFF | Seq16 |
| 17A-21MO-4|| | 6-6*01 or *03 | Not identified | Not identified | Not identified | |
| 17A-21MO-5 | 6-6*01 or *03 | 1*01 | 2-2*01 | CASSRQDTGELFF | Seq16 |
| 17A-21MO-6 | 6-6*01 | 1*01 | 2-2*01 | CASSRQDTGELFF | Seq16 |
| 17A-21MO-7 | 6-6*01 | 1*01 | 2-2*01 | CASSRQDTGELFF | Seq16 |
| 17A-21MO-8¶ | 5-1*01 | 1*01 | 1-2*01 | CASSLDGRANYGYTF | Seq17 |
| 17A-21MO-11¶ | 5-1*01 | 1*01 | 1-2*01 | CASSLDGRANYGYTF | Seq17 |
| 17A-21MO-12¶ | 5-1*01 | 1*01 | 1-2*01 | CASSLDGRANYGYTF | Seq17 |
| 32A-5 | 6-1*01 | 1*01 | 2-7*01 | CASSDGQPIYEQYF | Seq18 |
| 32A-14 | 6-1*01 | 1*01 | 2-7*01 | CASSDGQPIYEQYF | Seq18 |
| 32A-15 | 6-1*01 | 1*01 | 2-7*01 | CASSDGQPIYEQYF | Seq18 |
| 32A-16 | 6-1*01 | 1*01 | 2-7*01 | CASSDGQPIYEQYF | Seq18 |
| 32A-18 | 6-1*01 | 1*01 | 2-7*01 | CASSDGQPIYEQYF | Seq18 |
| 32A-21 | 6-1*01 | 1*01 | 2-7*01 | CASSDGQPIYEQYF | Seq18 |
Gene names and junction sequences were determined from nucleotide sequences using IMGT/V-QUEST version 3.3.4.50 IMGT/V-QUEST uses IMGT/Junction Analysis52 for analysis of the junction.
Sequences were given a numerical identifier for labeling purposes. Analyzed nucleotide sequences are in supplemental Table 6.
TCRB V-D-J genes and junction sequence for this clone were described previously.32
Sequence matched equally well to 20-1*01, *02, *04, *05, and *06 alleles.
17A-21MO-4 multiplex PCR product typed as TCRBV13 using the method and nomenclature in Akatsuka et al.46 This PCR product sequenced with the TCRBC primer whereas sequencing failed repeatedly with the TCRBV13 primer.
Clone has 2 TCRBV cDNA products. The second product was identified as TCRBV7-3*02, TCRBD2*01 or *02, TCRBJ2-3*01 and contains an out-of-frame junction.