HLA class II alleles evaluated for immune recognition by post-dUCBT patient PBMCs: mismatched alleles
| Patient no. . | HLA-DR . | B3† . | B4 . | B5 . | HLA-DQ . | HLA-DP . | |||
|---|---|---|---|---|---|---|---|---|---|
| B1 . | B1 . | B1 . | B1 . | B1 . | B1 . | ||||
| 1 | 04:02 | ||||||||
| 2 | 01:02‡(01:01) | 01:01 | 05:01 | 13:01 | |||||
| 3 | 02:01 | ||||||||
| 4 | 08:01 | 04:02 | 02:01 | ||||||
| 5 | 07:01 | 01:01‡(01:03) | 02:02§ | 10:01 | 15:01 | ||||
| 6 | 13:01 | ||||||||
| 7 | 06:01 | ||||||||
| 8 | 07:01 | 15:01 | 01:01 | 03:03 | 04:01 | ||||
| 9 | 04:04‡(04:01) | 15:01 | 01:01‡(01:03) | 01:01 | 03:02 | 06:02 | 04:01 | ||
| 10 | 11:04‡(11:01) | 02:02 | 03:01 | ||||||
| 11 | 15:01 | 01:01 | 06:02 | ||||||
| Patient no. . | HLA-DR . | B3† . | B4 . | B5 . | HLA-DQ . | HLA-DP . | |||
|---|---|---|---|---|---|---|---|---|---|
| B1 . | B1 . | B1 . | B1 . | B1 . | B1 . | ||||
| 1 | 04:02 | ||||||||
| 2 | 01:02‡(01:01) | 01:01 | 05:01 | 13:01 | |||||
| 3 | 02:01 | ||||||||
| 4 | 08:01 | 04:02 | 02:01 | ||||||
| 5 | 07:01 | 01:01‡(01:03) | 02:02§ | 10:01 | 15:01 | ||||
| 6 | 13:01 | ||||||||
| 7 | 06:01 | ||||||||
| 8 | 07:01 | 15:01 | 01:01 | 03:03 | 04:01 | ||||
| 9 | 04:04‡(04:01) | 15:01 | 01:01‡(01:03) | 01:01 | 03:02 | 06:02 | 04:01 | ||
| 10 | 11:04‡(11:01) | 02:02 | 03:01 | ||||||
| 11 | 15:01 | 01:01 | 06:02 | ||||||
Mismatched alleles between predominant CBU and nonengrafting UCB. Allelic variants of DRB1 are linked with either none or one of the paralogue genes DRB3, DRB4, and DRB5. There are related pseudogenes, DRB2, DRB6, DRB7, DRB8, and DRB9, that are not expressed. DRB1*01, DRB1*08, and DRB1*10 have no paralogue genes; DRB1*03 and DRB1*11/12/13/14 have paralogue gene DRB3; DRB1*04 and DRB1*07/09 have paralogue gene DRB4; and DRB1*15 and DRB1*16 have paralogue gene DRB5.
Although DRB1 is expressed at a level 5 times higher than its paralogs, we included in the analysis of a specific DRB1 mismatch allele the most probable DRB1 paralog as well.
Lack of relevant DNA sequences prohibited generation of allele-specific HeLa; most related available allele was used instead (in parentheses).
Generation of HLA-DQB1*02:02 expressing HeLa cell not successful; this allele was not tested.