Table 2 Frequency of PEST mutations in... | American Society of Hematology

Table 2

Frequency of PEST mutations in Kras^G12D-initiated T-ALL tumors complemented with MigR1 (control), myr-AKT, or Myc

Tumor	PEST mutation*	Mutational consequence†
Control-1	DelTACACA, InsAGGG @7282	Δ2340
Control-3	None‡	WT
Control-4	Deletion TGGTCCCACCCATGACC 7604	Δ2447
Control-5	Deletion CGGCTGGCAACACA 7345	Δ2361
Control-6	Insertion AGTACCCC 7513	Δ2416
Control-7	Insertion C 7524	Δ2420
Control-8	Insertion AA/Deletion G 7346	Δ2361
Control-9	Substitution C to G 7326	Δ2354
Control-10	Insertion GGGG 7460	Δ2399
myr-AKT-1	InsAGCC@7345	Δ2361
myr-AKT-2	DelG, InsCC@7346	Δ2361
myr-AKT-3	DelCA, InsGGGACCAA@7519	Δ2419
myr-AKT-4	InsCC@7346	Δ2361
myr-AKT-5	None	WT
myr-AKT-6	None‡	WT
myr-AKT-7	DelG, InsCA@7317	Δ2351
Myc-1	None	WT
Myc-2	None	WT
Myc-3	None	WT
Myc-4	None	WT
Myc-5	None	WT
Myc-6	None	WT
Myc-7	None	WT

Tumor	PEST mutation*	Mutational consequence†
Control-1	DelTACACA, InsAGGG @7282	Δ2340
Control-3	None‡	WT
Control-4	Deletion TGGTCCCACCCATGACC 7604	Δ2447
Control-5	Deletion CGGCTGGCAACACA 7345	Δ2361
Control-6	Insertion AGTACCCC 7513	Δ2416
Control-7	Insertion C 7524	Δ2420
Control-8	Insertion AA/Deletion G 7346	Δ2361
Control-9	Substitution C to G 7326	Δ2354
Control-10	Insertion GGGG 7460	Δ2399
myr-AKT-1	InsAGCC@7345	Δ2361
myr-AKT-2	DelG, InsCC@7346	Δ2361
myr-AKT-3	DelCA, InsGGGACCAA@7519	Δ2419
myr-AKT-4	InsCC@7346	Δ2361
myr-AKT-5	None	WT
myr-AKT-6	None‡	WT
myr-AKT-7	DelG, InsCA@7317	Δ2351
Myc-1	None	WT
Myc-2	None	WT
Myc-3	None	WT
Myc-4	None	WT
Myc-5	None	WT
Myc-6	None	WT
Myc-7	None	WT

Numbers correspond to nucleotide positions in Notch1 complementary DNA.

Numbers indicate amino acid residues in Notch1 at which mutations occur.

Truncated Notch1 protein was detected by western blot (Figure 5C).