Primer sequences used for Q-PCR of ECM proteins
| ECM component . | Forward . | Reverse . |
|---|---|---|
| Collagen I | TGACTGGAAGAGCGGAGAGTACT | CCTTGATGGCGTCCAGGTT |
| Collagen III | CTGTAACATGGAAACTGGGGAAA | CCATAGCTGAACTGAAAACCACC |
| Collagen IV | AACAACGTCTGCAACTTCGC | CTTCACAAACCGCACACCTG |
| Collagen V | CTTCGCCGCTACTCCTGTTC | CCCTGAGGGCAAATTGTGAAAA |
| Collagen VI | CTGCTGCTACAAGCCTGCT | CCCCATAAGGTTTCAGCCTCA |
| FN | GAGAGGAGCACTACCCCAGA | GCCCGGATTAAGGTTGGTGA |
| Decorin | AATGTGGGTGTCAGCTGGAT | CTAGCAAGGTTGTGTCGGGT |
| ECM component . | Forward . | Reverse . |
|---|---|---|
| Collagen I | TGACTGGAAGAGCGGAGAGTACT | CCTTGATGGCGTCCAGGTT |
| Collagen III | CTGTAACATGGAAACTGGGGAAA | CCATAGCTGAACTGAAAACCACC |
| Collagen IV | AACAACGTCTGCAACTTCGC | CTTCACAAACCGCACACCTG |
| Collagen V | CTTCGCCGCTACTCCTGTTC | CCCTGAGGGCAAATTGTGAAAA |
| Collagen VI | CTGCTGCTACAAGCCTGCT | CCCCATAAGGTTTCAGCCTCA |
| FN | GAGAGGAGCACTACCCCAGA | GCCCGGATTAAGGTTGGTGA |
| Decorin | AATGTGGGTGTCAGCTGGAT | CTAGCAAGGTTGTGTCGGGT |
Q-PCR, quantitative PCR.