SPR analysis of fibrinogen variant binding to immobilized FXIII-A2B2 and rFXIII-B2
| Immobilized ligand . | Analyte . | N . | Equilibrium dissociation constant (KD), nM . |
|---|---|---|---|
| FXIII-A2B2 | γA/γA | 6 | 3.8 ± 2.4 |
| γʹ/γʹ | 6 | 10.4 ± 11.1 | |
| Aα251 | 3 | 71.0 ± 16.2 | |
| Fibγ390-396A | 6 | No binding | |
| rFXIII-B2 | γA/γA | 4 | 0.4 ± 0.3 |
| γʹ/γʹ | 6 | 53.0 ± 75.1 | |
| Aα251 | 4 | 58.6 ± 26.5 | |
| Fibγ390-396A | 6 | No binding |
| Immobilized ligand . | Analyte . | N . | Equilibrium dissociation constant (KD), nM . |
|---|---|---|---|
| FXIII-A2B2 | γA/γA | 6 | 3.8 ± 2.4 |
| γʹ/γʹ | 6 | 10.4 ± 11.1 | |
| Aα251 | 3 | 71.0 ± 16.2 | |
| Fibγ390-396A | 6 | No binding | |
| rFXIII-B2 | γA/γA | 4 | 0.4 ± 0.3 |
| γʹ/γʹ | 6 | 53.0 ± 75.1 | |
| Aα251 | 4 | 58.6 ± 26.5 | |
| Fibγ390-396A | 6 | No binding |
Equilibrium dissociation constant values are mean ± standard deviation for the number of experiments indicated (N).