Thromboelastometric properties of fibrin clots produced with whole blood, normal pooled plasma, and fibrinogen-deficient plasma supplemented with γA/γA and γA/γ′ fibrinogen
| . | Whole blood . | Normal pooled plasma . | Fibrinogen-deficient plasma . | |||||
|---|---|---|---|---|---|---|---|---|
| γA/γA . | γA/γ′ . | |||||||
| Thrombin, U/mL | 1.0 | 10.0 | 1.0 | 10.0 | 1.0 | 10.0 | 1.0 | 10.0 |
| MC, mm | 60.0 ± 3.6 | 36.0 ± 11.1* | 25.7 ± 3.0 | 11.2 ± 3.3* | 20.5 ± 1.3 | 13.7 ± 4.2* | 12.7 ± 0.6# | 11.3 ± 2.1 |
| G, ×103, dyne/cm2 | 7.7 ± 1.1 | 3.0 ± 1.3* | 1.7 ± 0.3 | 0.6 ± 0.2* | 1.3 ± 0.1 | 0.8 ± 0.3* | 0.7 ± 0.0# | 0.6 ± 0.1 |
| CT, s | 81.5 ± 27.6 | 46.0 ± 27.7 | 76.7 ± 23.9 | 19.8 ± 10.5* | 131.3 ± 29.3 | 11.7 ± 5.9* | 204.3 ± 8.7# | 13.7 ± 5.5* |
| CFR, ° | 65.0 ± 5.6 | 59.7 ± 6.4 | 53.3 ± 8.0 | 58.3 ± 4.1 | 58.5 ± 1.7 | 71.0 ± 10.4 | 44.0 ± 5.3 | 59.3 ± 6.5 |
| . | Whole blood . | Normal pooled plasma . | Fibrinogen-deficient plasma . | |||||
|---|---|---|---|---|---|---|---|---|
| γA/γA . | γA/γ′ . | |||||||
| Thrombin, U/mL | 1.0 | 10.0 | 1.0 | 10.0 | 1.0 | 10.0 | 1.0 | 10.0 |
| MC, mm | 60.0 ± 3.6 | 36.0 ± 11.1* | 25.7 ± 3.0 | 11.2 ± 3.3* | 20.5 ± 1.3 | 13.7 ± 4.2* | 12.7 ± 0.6# | 11.3 ± 2.1 |
| G, ×103, dyne/cm2 | 7.7 ± 1.1 | 3.0 ± 1.3* | 1.7 ± 0.3 | 0.6 ± 0.2* | 1.3 ± 0.1 | 0.8 ± 0.3* | 0.7 ± 0.0# | 0.6 ± 0.1 |
| CT, s | 81.5 ± 27.6 | 46.0 ± 27.7 | 76.7 ± 23.9 | 19.8 ± 10.5* | 131.3 ± 29.3 | 11.7 ± 5.9* | 204.3 ± 8.7# | 13.7 ± 5.5* |
| CFR, ° | 65.0 ± 5.6 | 59.7 ± 6.4 | 53.3 ± 8.0 | 58.3 ± 4.1 | 58.5 ± 1.7 | 71.0 ± 10.4 | 44.0 ± 5.3 | 59.3 ± 6.5 |
Data represented as mean ± SD; n = 3. Statistical significance is denoted with *P < .05 for differences between 1.0 and 10.0 U/mL thrombin and #P < .05 for comparison between γA/γA and γA/γ′ supplemented fibrinogen-deficient plasmas with the same thrombin concentration. CFR, clot formation rate; CT, clot formation time; G, storage modulus; MCF, maximum clot firmness.