Raw 264.7 macrophages heme-oxygenase activity assay
| Treatment . | pmol Bilirubin/mg microsomal protein/hour . |
|---|---|
| NT | 181.165 ± 20.091 |
| Hx-heme | 183.977 ± 10.261 |
| HSA-heme | 264.141 ± 5.582 vs NT; vs Hx-heme **P < .01 |
| MSA-heme | 246.633 ± 11.161 vs NT; vs Hx-heme *P < .05 |
| Heme | 376.744 ± 11.083 vs NT; vs Hx-heme *P < .05 |
| Treatment . | pmol Bilirubin/mg microsomal protein/hour . |
|---|---|
| NT | 181.165 ± 20.091 |
| Hx-heme | 183.977 ± 10.261 |
| HSA-heme | 264.141 ± 5.582 vs NT; vs Hx-heme **P < .01 |
| MSA-heme | 246.633 ± 11.161 vs NT; vs Hx-heme *P < .05 |
| Heme | 376.744 ± 11.083 vs NT; vs Hx-heme *P < .05 |
The assay was performed on microsomes purified from Raw 264.7 cells untreated (NT) or treated with 15 μM Hx-heme, HSA-heme, MSA-heme, or heme alone for 5 hours. Values represent mean ± SEM. *P < .05; **P < .01; ***P < .001. n = 4 for each sample.