Combination indices of AZD6738 (1 µM) with cytotoxic chemotherapy in CII-ATMsh and Mec1 cells
| Drug combined with AZD6738 and dose (µM) . | CII-ATMsh . | Mec1 . | ||||
|---|---|---|---|---|---|---|
| Fraction affected . | Combination index (mean ± SEM) . | Synergism* . | Fraction affected . | Combination index (mean ± SEM) . | Synergism* . | |
| Chlorambucil | ||||||
| 1 | 0.43 | 0.45 ± 0.16 | +++ | 0.51 | 0.75 ± 0.16 | ++ |
| 2.5 | 0.54 | 0.48 ± 0.09 | +++ | 0.61 | 0.49 ± 0.11 | +++ |
| 5 | 0.73 | 0.27 ± 0.14 | ++++ | 0.71 | 0.29 ± 0.03 | ++++ |
| 10 | 0.89 | 0.16 ± 0.08 | ++++ | 0.76 | 0.26 ± 0.03 | ++++ |
| Fludarabine | ||||||
| 1 | 0.35 | 0.89 ± 0.29 | + | 0.44 | 1.06 ± 0.14 | ± |
| 2.5 | 0.37 | 0.93 ± 0.28 | ± | 0.55 | 0.69 ± 0.14 | +++ |
| 5 | 0.51 | 0.69 ± 0.17 | +++ | 0.61 | 0.56 ± 0.18 | +++ |
| 10 | 0.71 | 0.55 ± 0.13 | +++ | 0.72 | 0.34 ± 0.07 | +++ |
| 4-Hydroperoxycyclophosphamide | ||||||
| 0.25 | 0.40 | 0.75 ± 0.33 | ++ | 0.48 | 0.97 ± 0.30 | ± |
| 0.5 | 0.56 | 0.53 ± 0.26 | +++ | 0.57 | 0.69 ± 0.28 | +++ |
| 1 | 0.73 | 0.36 ± 0.15 | +++ | 0.68 | 0.42 ± 0.14 | +++ |
| 2 | 0.89 | 0.30 ± 0.07 | ++++ | 0.78 | 0.26 ± 0.10 | ++++ |
| Bendamustine | ||||||
| 10 | 0.24 | 1.08 ± 0.15 | ± | 0.39 | 1.10 ± 0.08 | ± |
| 25 | 0.31 | 0.86 ± 0.15 | + | 0.49 | 0.89 ± 0.29 | + |
| 50 | 0.40 | 0.66 ± 0.15 | +++ | 0.52 | 0.76 ± 0.18 | ++ |
| 100 | 0.65 | 0.34 ± 0.11 | +++ | 0.66 | 0.41 ± 0.01 | +++ |
| Drug combined with AZD6738 and dose (µM) . | CII-ATMsh . | Mec1 . | ||||
|---|---|---|---|---|---|---|
| Fraction affected . | Combination index (mean ± SEM) . | Synergism* . | Fraction affected . | Combination index (mean ± SEM) . | Synergism* . | |
| Chlorambucil | ||||||
| 1 | 0.43 | 0.45 ± 0.16 | +++ | 0.51 | 0.75 ± 0.16 | ++ |
| 2.5 | 0.54 | 0.48 ± 0.09 | +++ | 0.61 | 0.49 ± 0.11 | +++ |
| 5 | 0.73 | 0.27 ± 0.14 | ++++ | 0.71 | 0.29 ± 0.03 | ++++ |
| 10 | 0.89 | 0.16 ± 0.08 | ++++ | 0.76 | 0.26 ± 0.03 | ++++ |
| Fludarabine | ||||||
| 1 | 0.35 | 0.89 ± 0.29 | + | 0.44 | 1.06 ± 0.14 | ± |
| 2.5 | 0.37 | 0.93 ± 0.28 | ± | 0.55 | 0.69 ± 0.14 | +++ |
| 5 | 0.51 | 0.69 ± 0.17 | +++ | 0.61 | 0.56 ± 0.18 | +++ |
| 10 | 0.71 | 0.55 ± 0.13 | +++ | 0.72 | 0.34 ± 0.07 | +++ |
| 4-Hydroperoxycyclophosphamide | ||||||
| 0.25 | 0.40 | 0.75 ± 0.33 | ++ | 0.48 | 0.97 ± 0.30 | ± |
| 0.5 | 0.56 | 0.53 ± 0.26 | +++ | 0.57 | 0.69 ± 0.28 | +++ |
| 1 | 0.73 | 0.36 ± 0.15 | +++ | 0.68 | 0.42 ± 0.14 | +++ |
| 2 | 0.89 | 0.30 ± 0.07 | ++++ | 0.78 | 0.26 ± 0.10 | ++++ |
| Bendamustine | ||||||
| 10 | 0.24 | 1.08 ± 0.15 | ± | 0.39 | 1.10 ± 0.08 | ± |
| 25 | 0.31 | 0.86 ± 0.15 | + | 0.49 | 0.89 ± 0.29 | + |
| 50 | 0.40 | 0.66 ± 0.15 | +++ | 0.52 | 0.76 ± 0.18 | ++ |
| 100 | 0.65 | 0.34 ± 0.11 | +++ | 0.66 | 0.41 ± 0.01 | +++ |
++++, strong synergism; +++, synergism; ++, moderate synergism; +, slight synergism; ±, additive.