qRT-PCR of mast cell progenitors
| Target gene . | HMC1 . | MCp1 . | MCp2 . | MCp3 . |
|---|---|---|---|---|
| Cma1 | 29 (4/4) | ud (0/3) | ud (0/4) | ud (0/3) |
| Cpa3 | 20 (4/4) | ud (0/3) | 33 (3/4) | ud (0/3) |
| Tpsab1/Tpsb2 | 14 (4/4) | 32 (2/3) | 33 (3/4) | 32 (1/3) |
| Gzmb | 22 (4/4) | ud (0/3) | 37 (1/4) | ud (0/3) |
| Srgn | 16 (4/4) | 32 (2/3) | 32 (4/4) | 33 (3/3) |
| Actb | 11 (3/3) | 32 (2/3) | 31 (3/3) | 32 (2/3) |
| Gapdh | 14 (4/4) | 35 (1/3) | 34 (3/4) | ud (0/3) |
| Target gene . | HMC1 . | MCp1 . | MCp2 . | MCp3 . |
|---|---|---|---|---|
| Cma1 | 29 (4/4) | ud (0/3) | ud (0/4) | ud (0/3) |
| Cpa3 | 20 (4/4) | ud (0/3) | 33 (3/4) | ud (0/3) |
| Tpsab1/Tpsb2 | 14 (4/4) | 32 (2/3) | 33 (3/4) | 32 (1/3) |
| Gzmb | 22 (4/4) | ud (0/3) | 37 (1/4) | ud (0/3) |
| Srgn | 16 (4/4) | 32 (2/3) | 32 (4/4) | 33 (3/3) |
| Actb | 11 (3/3) | 32 (2/3) | 31 (3/3) | 32 (2/3) |
| Gapdh | 14 (4/4) | 35 (1/3) | 34 (3/4) | ud (0/3) |
mRNA levels presented as raw CT values . Lin− CD34hi CD117int/hi FcεRI+ mast cell progenitors from 3 donors (MCp1-3) and HMC1 cells were subjected to qRT-PCR for mast cell proteases (Cma1, Cpa3, Tpsab1/Tpsb2, Gzmb), serglycin (Srgn), and the housekeeping genes β-actin (Actb) and glyceraldehyde 3-phosphate dehydrogenase (Gapdh). The mean CT values for 3 to 4 qRT-PCR analyses of the same samples are presented. The number of qRT-PCR analyses that contributed to the total number of analyses of each gene is presented in parentheses after the mean CT number. The controls without cDNA did not generate detectable signals for any of the target genes, except for Actb where the mean background CT value was 36.
ud, under detection limit in all runs.