Identified glycoproteins which were significantly increased according to their relative increase between TLR9 agonist–treated and –nontreated (Con) ZAP-70–positive CLL cells of 3 different patients
| Protein name . | Gene name . | Unique peptides . | Mean DSP30/Con . | P . |
|---|---|---|---|---|
| α-1,6-mannosylglycoprotein 6-β-N-acetylglucosaminyltransferase A | MGAT5 | 14 | 36.12 | 2.45E-02 |
| V-type proton ATPase subunit S1 | ATP6AP1 | 4 | 29.92 | 2.25E-02 |
| Protein CutA | CUTA | 3 | 29.15 | 2.06E-02 |
| Ig μ chain C region | IGHM | 10 | 24.22 | 9.63E-03 |
| Serglycin | SRGN | 6 | 12.98 | 6.83E-05 |
| 4F2 cell-surface antigen heavy chain | SLC3A2 | 10 | 12.38 | 5.67E-04 |
| Hypoxia upregulated protein 1 | HYOU1 | 5 | 12.06 | 4.74E-02 |
| Platelet-derived growth factor D | PDGFD | 5 | 9.59 | 3.23E-02 |
| l-selectin | SELL | 4 | 9.27 | 1.00E-02 |
| α-mannosidase 2 | MAN2A1 | 16 | 9.25 | 5.96E-04 |
| Fibromodulin | FMOD | 6 | 8.82 | 5.15E-03 |
| Attractin | ATRN | 8 | 8.69 | 7.30E-05 |
| HLA class I histocompatibility antigen, α chain E | HLA-E | 2 | 7.93 | 4.54E-02 |
| Transforming growth factor β receptor type 3 | TGFBR3 | 3 | 7.45 | 2.05E-04 |
| Receptor-type tyrosine-protein phosphatase η | PTPRJ | 7 | 7.36 | 3.59E-03 |
| CD44 antigen | CD44 | 10 | 6.78 | 4.87E-03 |
| Sulfhydryl oxidase 1 | QSOX1 | 16 | 6.72 | 4.28E-02 |
| Granulins | GRN | 16 | 6.67 | 4.04E-04 |
| Ig λ-2 chain C regions | IGLC2 | 3 | 6.21 | 5.29E-04 |
| Adipocyte enhancer-binding protein 1 | AEBP1 | 2 | 5.95 | 1.63E-02 |
| Calsyntenin-1 | CLSTN1 | 20 | 5.47 | 2.23E-03 |
| Semaphorin-7A | SEMA7A | 7 | 5.39 | 2.03E-03 |
| Dystroglycan | DAG1 | 4 | 5.17 | 6.43E-04 |
| Fc receptor-like protein 5 | FCRL5 | 7 | 4.93 | 1.14E-03 |
| Golgi apparatus protein 1 | GLG1 | 16 | 4.80 | 4.42E-02 |
| β-galactoside α-2,6-sialyltransferase 1 | ST6GAL1 | 7 | 4.54 | 4.07E-03 |
| Glucosidase 2 subunit β | PRKCSH | 6 | 4.19 | 1.39E-02 |
| CD48 antigen | CD48 | 4 | 3.94 | 1.81E-02 |
| Calsyntenin-3 | CLSTN3 | 11 | 3.72 | 1.99E-02 |
| Amyloid β A4 protein | APP | 12 | 3.40 | 4.80E-03 |
| Leucyl-cystinyl aminopeptidase, pregnancy serum form | LNPEP | 7 | 3.25 | 2.20E-03 |
| Endoplasmic reticulum aminopeptidase 1 | ERAP1 | 8 | 2.95 | 3.85E-02 |
| Neuroserpin | SERPINI1 | 3 | 2.51 | 3.14E-02 |
| CD82 antigen | CD82 | 3 | 0.42 | 3.60E-03 |
| SPARC | SPARC | 2 | 0.37 | 3.81E-03 |
| Xaa-Pro dipeptidase | PEPD | 6 | 0.29 | 9.21E-04 |
| C-X-C chemokine receptor type 4 | CXCR4 | 3 | 0.28 | 4.91E-02 |
| Glycogenin-1 | GYG1 | 3 | 0.10 | 2.32E-02 |
| Protein name . | Gene name . | Unique peptides . | Mean DSP30/Con . | P . |
|---|---|---|---|---|
| α-1,6-mannosylglycoprotein 6-β-N-acetylglucosaminyltransferase A | MGAT5 | 14 | 36.12 | 2.45E-02 |
| V-type proton ATPase subunit S1 | ATP6AP1 | 4 | 29.92 | 2.25E-02 |
| Protein CutA | CUTA | 3 | 29.15 | 2.06E-02 |
| Ig μ chain C region | IGHM | 10 | 24.22 | 9.63E-03 |
| Serglycin | SRGN | 6 | 12.98 | 6.83E-05 |
| 4F2 cell-surface antigen heavy chain | SLC3A2 | 10 | 12.38 | 5.67E-04 |
| Hypoxia upregulated protein 1 | HYOU1 | 5 | 12.06 | 4.74E-02 |
| Platelet-derived growth factor D | PDGFD | 5 | 9.59 | 3.23E-02 |
| l-selectin | SELL | 4 | 9.27 | 1.00E-02 |
| α-mannosidase 2 | MAN2A1 | 16 | 9.25 | 5.96E-04 |
| Fibromodulin | FMOD | 6 | 8.82 | 5.15E-03 |
| Attractin | ATRN | 8 | 8.69 | 7.30E-05 |
| HLA class I histocompatibility antigen, α chain E | HLA-E | 2 | 7.93 | 4.54E-02 |
| Transforming growth factor β receptor type 3 | TGFBR3 | 3 | 7.45 | 2.05E-04 |
| Receptor-type tyrosine-protein phosphatase η | PTPRJ | 7 | 7.36 | 3.59E-03 |
| CD44 antigen | CD44 | 10 | 6.78 | 4.87E-03 |
| Sulfhydryl oxidase 1 | QSOX1 | 16 | 6.72 | 4.28E-02 |
| Granulins | GRN | 16 | 6.67 | 4.04E-04 |
| Ig λ-2 chain C regions | IGLC2 | 3 | 6.21 | 5.29E-04 |
| Adipocyte enhancer-binding protein 1 | AEBP1 | 2 | 5.95 | 1.63E-02 |
| Calsyntenin-1 | CLSTN1 | 20 | 5.47 | 2.23E-03 |
| Semaphorin-7A | SEMA7A | 7 | 5.39 | 2.03E-03 |
| Dystroglycan | DAG1 | 4 | 5.17 | 6.43E-04 |
| Fc receptor-like protein 5 | FCRL5 | 7 | 4.93 | 1.14E-03 |
| Golgi apparatus protein 1 | GLG1 | 16 | 4.80 | 4.42E-02 |
| β-galactoside α-2,6-sialyltransferase 1 | ST6GAL1 | 7 | 4.54 | 4.07E-03 |
| Glucosidase 2 subunit β | PRKCSH | 6 | 4.19 | 1.39E-02 |
| CD48 antigen | CD48 | 4 | 3.94 | 1.81E-02 |
| Calsyntenin-3 | CLSTN3 | 11 | 3.72 | 1.99E-02 |
| Amyloid β A4 protein | APP | 12 | 3.40 | 4.80E-03 |
| Leucyl-cystinyl aminopeptidase, pregnancy serum form | LNPEP | 7 | 3.25 | 2.20E-03 |
| Endoplasmic reticulum aminopeptidase 1 | ERAP1 | 8 | 2.95 | 3.85E-02 |
| Neuroserpin | SERPINI1 | 3 | 2.51 | 3.14E-02 |
| CD82 antigen | CD82 | 3 | 0.42 | 3.60E-03 |
| SPARC | SPARC | 2 | 0.37 | 3.81E-03 |
| Xaa-Pro dipeptidase | PEPD | 6 | 0.29 | 9.21E-04 |
| C-X-C chemokine receptor type 4 | CXCR4 | 3 | 0.28 | 4.91E-02 |
| Glycogenin-1 | GYG1 | 3 | 0.10 | 2.32E-02 |
The Student t test value was calculated from the log2-transformed fold changes between control and DSP30 with a heteroscedastic Student t test.