Advantages and disadvantages of different rapid culture techniques
| Method . | Advantages . | Disadvantages . |
|---|---|---|
| Short ex vivo culture | Expand low frequency VSTs; not restricted by HLA type | Still 10-14 d culture period; not yet available when donor seronegative |
| Multimer selection | Rapid manufacturing; already in late phase trials | Restricted to certain HLA types; few class II multimers available; not available when donor seronegative or has low frequency of circulating T cells specific for the peptides |
| γ capture | Rapid manufacturing; not restricted to certain HLA types; will select polyclonal T cells recognizing multiple epitopes | Large volume of blood required; will not select T cells producing other cytokines; may still require ex vivo culture to expand; not available when donor seronegative |
| Third-party T cells | Immediately available; available for patients with seronegative donors | Shorter persistence; response rate likely lower; not available for rare HLA types |
| Method . | Advantages . | Disadvantages . |
|---|---|---|
| Short ex vivo culture | Expand low frequency VSTs; not restricted by HLA type | Still 10-14 d culture period; not yet available when donor seronegative |
| Multimer selection | Rapid manufacturing; already in late phase trials | Restricted to certain HLA types; few class II multimers available; not available when donor seronegative or has low frequency of circulating T cells specific for the peptides |
| γ capture | Rapid manufacturing; not restricted to certain HLA types; will select polyclonal T cells recognizing multiple epitopes | Large volume of blood required; will not select T cells producing other cytokines; may still require ex vivo culture to expand; not available when donor seronegative |
| Third-party T cells | Immediately available; available for patients with seronegative donors | Shorter persistence; response rate likely lower; not available for rare HLA types |