Individual Treg product phenotype of non-CD4+FoxP3+CD127low cells and cytokine profile CD4+FoxP3+CD127low and non-CD4+FoxP3+CD127low cells
| . | Infused product . | Noninfused products . | Median all products . | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Product No. . | 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 9 . | 10 . | 11 . | 12 . | 13 . | |
| Phenotype | ||||||||||||||
| CD4–CD8++ | 0.14 | 0.06 | 0.80 | 0.04 | 0.04 | 0.77 | 1.36 | 0.23 | 0.06 | 0.88 | 0.03 | NA | 0.13 | 0.14 |
| CD4+CD8+ | 0.18 | 0.48 | 0.55 | 0.07 | 0.07 | 1.19 | 0.18 | 1.55 | 0.53 | 0.84 | 5.00 | NA | 2.40 | 0.54 |
| CD14+ | NA | NA | NA | NA | NA | 0.02 | 0.00 | 0.00 | 0.01 | 0.02 | 0.03 | NA | 0.08 | 0.02 |
| CD19+ | 0.06 | 0.44 | 0.08 | 0.11 | 0.01 | 0.03 | 0.00 | 0.00 | 0.00 | 0.00 | 0.02 | NA | 0.07 | 0.03 |
| CD4+FoxP3+ | ||||||||||||||
| HELIOS+ | 93.9 | 88.3 | 85.6 | 92.7 | 95.7 | NA | NA | NA | NA | 66.8 | 98.5 | NA | 97.6 | 89.9 |
| KI-67+ | 5.5 | 7.4 | 4.0 | 4.6 | 6.7 | 3.7 | 1.8 | 3.0 | 4.3 | 9.7 | 4.4 | NA | 16.7 | 6.0 |
| CD4+Foxp3– | ||||||||||||||
| HELIOS+ | 72.9 | 55.4 | 65.0 | 34.5 | 59.0 | NA | NA | NA | NA | 72.0 | 95.4 | NA | 90.2 | 68.1 |
| KI-67– | 3.4 | 2.3 | 1.4 | 1.4 | 2.4 | 0.6 | 0.0 | 3.4 | 1.3 | 11.2 | 2.4 | NA | 15.4 | 3.8 |
| Cytokine expression | ||||||||||||||
| CD4+Foxp3+Helios+ | ||||||||||||||
| IL2+ | 3.70 | 3.83 | 4.64 | 4.12 | 3.57 | 2.37 | 5.05 | 5.48 | 2.18 | 2.71 | 3.42 | 0.11 | NA | 3.6 |
| IFNγ+ | 0.55 | 0.26 | 0.39 | 0.55 | 0.88 | 0.59 | 0.67 | 3.18 | 0.47 | 0.92 | 0.39 | 0.25 | NA | 0.6 |
| TNFα+ | 27.50 | 28.90 | 21.20 | 15.80 | 43.40 | 41.20 | 34.90 | 67.90 | 70.50 | 20.10 | 27.90 | 4.30 | NA | 28.4 |
| IL4+ | 2.33 | 1.48 | 1.00 | 1.05 | 1.18 | 0.81 | 3.84 | 2.41 | 0.39 | 1.84 | 1.04 | 0.02 | NA | 1.1 |
| IL10+ | 0.19 | 0.14 | 0.11 | 1.79 | 0.28 | 0.38 | 1.18 | 2.52 | 0.86 | 0.28 | 0.42 | 0.26 | NA | 0.3 |
| IL17+ | 1.02 | 0.19 | 0.23 | 0.77 | 1.40 | 0.78 | 0.84 | 2.08 | 0.39 | 0.26 | 0.25 | 0.03 | NA | 0.6 |
| CD4+Foxp3–Helios– | ||||||||||||||
| IL2+ | 0.40 | 0.15 | 0.09 | 0.61 | 0.08 | 0.04 | 0.14 | 0.15 | 0.04 | 0.15 | 0.11 | 0.27 | NA | 0.1 |
| IFN+ | 0.19 | 0.15 | 0.04 | 0.99 | 0.18 | 0.09 | 0.14 | 1.06 | 0.22 | 0.07 | 0.30 | 0.05 | NA | 0.2 |
| TNFα+ | 4.57 | 2.62 | 4.76 | 3.15 | 2.77 | 5.41 | 8.09 | 12.90 | 15.20 | 3.98 | 3.02 | 0.40 | NA | 4.3 |
| IL4+ | 0.12 | 0.29 | 0.07 | 0.19 | 0.14 | 0.15 | 0.11 | 0.89 | 0.11 | 0.06 | 0.19 | 0.01 | NA | 0.1 |
| IL10+ | 0.09 | 0.02 | 0.00 | 0.12 | 0.05 | 0.06 | 0.21 | 0.35 | 0.29 | 0.08 | 0.04 | 0.08 | NA | 0.1 |
| IL17+ | 0.24 | 0.19 | 0.02 | 0.20 | 0.24 | 0.15 | 0.20 | 1.33 | 0.18 | 0.02 | 0.15 | 0.01 | NA | 0.2 |
| . | Infused product . | Noninfused products . | Median all products . | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Product No. . | 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 9 . | 10 . | 11 . | 12 . | 13 . | |
| Phenotype | ||||||||||||||
| CD4–CD8++ | 0.14 | 0.06 | 0.80 | 0.04 | 0.04 | 0.77 | 1.36 | 0.23 | 0.06 | 0.88 | 0.03 | NA | 0.13 | 0.14 |
| CD4+CD8+ | 0.18 | 0.48 | 0.55 | 0.07 | 0.07 | 1.19 | 0.18 | 1.55 | 0.53 | 0.84 | 5.00 | NA | 2.40 | 0.54 |
| CD14+ | NA | NA | NA | NA | NA | 0.02 | 0.00 | 0.00 | 0.01 | 0.02 | 0.03 | NA | 0.08 | 0.02 |
| CD19+ | 0.06 | 0.44 | 0.08 | 0.11 | 0.01 | 0.03 | 0.00 | 0.00 | 0.00 | 0.00 | 0.02 | NA | 0.07 | 0.03 |
| CD4+FoxP3+ | ||||||||||||||
| HELIOS+ | 93.9 | 88.3 | 85.6 | 92.7 | 95.7 | NA | NA | NA | NA | 66.8 | 98.5 | NA | 97.6 | 89.9 |
| KI-67+ | 5.5 | 7.4 | 4.0 | 4.6 | 6.7 | 3.7 | 1.8 | 3.0 | 4.3 | 9.7 | 4.4 | NA | 16.7 | 6.0 |
| CD4+Foxp3– | ||||||||||||||
| HELIOS+ | 72.9 | 55.4 | 65.0 | 34.5 | 59.0 | NA | NA | NA | NA | 72.0 | 95.4 | NA | 90.2 | 68.1 |
| KI-67– | 3.4 | 2.3 | 1.4 | 1.4 | 2.4 | 0.6 | 0.0 | 3.4 | 1.3 | 11.2 | 2.4 | NA | 15.4 | 3.8 |
| Cytokine expression | ||||||||||||||
| CD4+Foxp3+Helios+ | ||||||||||||||
| IL2+ | 3.70 | 3.83 | 4.64 | 4.12 | 3.57 | 2.37 | 5.05 | 5.48 | 2.18 | 2.71 | 3.42 | 0.11 | NA | 3.6 |
| IFNγ+ | 0.55 | 0.26 | 0.39 | 0.55 | 0.88 | 0.59 | 0.67 | 3.18 | 0.47 | 0.92 | 0.39 | 0.25 | NA | 0.6 |
| TNFα+ | 27.50 | 28.90 | 21.20 | 15.80 | 43.40 | 41.20 | 34.90 | 67.90 | 70.50 | 20.10 | 27.90 | 4.30 | NA | 28.4 |
| IL4+ | 2.33 | 1.48 | 1.00 | 1.05 | 1.18 | 0.81 | 3.84 | 2.41 | 0.39 | 1.84 | 1.04 | 0.02 | NA | 1.1 |
| IL10+ | 0.19 | 0.14 | 0.11 | 1.79 | 0.28 | 0.38 | 1.18 | 2.52 | 0.86 | 0.28 | 0.42 | 0.26 | NA | 0.3 |
| IL17+ | 1.02 | 0.19 | 0.23 | 0.77 | 1.40 | 0.78 | 0.84 | 2.08 | 0.39 | 0.26 | 0.25 | 0.03 | NA | 0.6 |
| CD4+Foxp3–Helios– | ||||||||||||||
| IL2+ | 0.40 | 0.15 | 0.09 | 0.61 | 0.08 | 0.04 | 0.14 | 0.15 | 0.04 | 0.15 | 0.11 | 0.27 | NA | 0.1 |
| IFN+ | 0.19 | 0.15 | 0.04 | 0.99 | 0.18 | 0.09 | 0.14 | 1.06 | 0.22 | 0.07 | 0.30 | 0.05 | NA | 0.2 |
| TNFα+ | 4.57 | 2.62 | 4.76 | 3.15 | 2.77 | 5.41 | 8.09 | 12.90 | 15.20 | 3.98 | 3.02 | 0.40 | NA | 4.3 |
| IL4+ | 0.12 | 0.29 | 0.07 | 0.19 | 0.14 | 0.15 | 0.11 | 0.89 | 0.11 | 0.06 | 0.19 | 0.01 | NA | 0.1 |
| IL10+ | 0.09 | 0.02 | 0.00 | 0.12 | 0.05 | 0.06 | 0.21 | 0.35 | 0.29 | 0.08 | 0.04 | 0.08 | NA | 0.1 |
| IL17+ | 0.24 | 0.19 | 0.02 | 0.20 | 0.24 | 0.15 | 0.20 | 1.33 | 0.18 | 0.02 | 0.15 | 0.01 | NA | 0.2 |
CD, cluster of differentiation; FoxP3, forkhead box P3; IFNγ, interferon-γ; IL, interleukin; NA, not available; TNAα, tumor necrosis factor-α; Treg, regulatory T cells.