Table 5 Association of CXCR3 T-cell and... | American Society of Hematology

Table 5

Association of CXCR3⁺ T-cell and NK-cell populations with plasma CXCL10 and CXCL9 in a subpopulation

Chemokine	Controls (n = 67/65)*		cGVHD cases (n = 52/44)*		P	ROC AUC
Chemokine	Mean	SD	Mean	SD	P	ROC AUC
Replication set 1
CXCL10, pg/mL	129	105	607	2194	.04	0.61
CXCL9, pg/mL	8969	9218	17715	18675	.02	0.63
Subset (% of parent population)†
CD3^–CD56^brightCXCR3⁺	12.6	10.8	10.3	11.5	.03	0.62
CD8⁺CXCR3⁺	23.4	12.9	22.1	12.8	.50	0.54
CD4⁺CXCR3⁺	13.2	8.5	11.0	8.2	.10	0.59
Ratio CXCL10/%
CD3^–CD56^brightCXCR3⁺	24.3	37.8	159	447	.03	0.63
CD8⁺CXCR3⁺	7.9	9.2	35.2	103.2	.13	0.58
CD4⁺CXCR3⁺	17.9	25.7	154	610	.04	0.61
Ratio CXCL9/%
CD3^–CD56^brightCXCR3⁺	1760	3090	7079	13023	.008	0.65
CD8⁺CXCR3⁺	574	808	1256	1570	.04	0.62
CD4⁺CXCR3⁺	1246	1883	3065	4870	.01	0.64
Replication set 2	(n = 25)		(n = 24)
CXCL10, pg/mL	238.6	449.4	819.5	1370	.005	0.73
CXCL9, pg/mL	16036	25116	25805	31451	.12	0.63
Subset (% of parent population)†
CD3^–CD56^brightCXCR3⁺	16.0	10.62	9.37	5.73	.01	0.70
CD8⁺CXCR3⁺	25.64	15.02	25.39	12.69	.98	0.51
CD4⁺CXCR3⁺	2.38 ± 2.43		1.90	2.32	.38	0.57
Ratio CXCL10/%
CD3^–CD56^brightCXCR3⁺	19.08	31.48	143.0	201.9	.0009	0.78
CD8⁺CXCR3⁺	14.43	33.96	48.6	113.7	.006	0.73
CD4⁺CXCR3⁺	223.3	336.1	1087	2371	.008	0.72
Ratio CXCL9/%
CD3^–CD56^brightCXCR3⁺	1155	1350	5887	11457	.05	0.67
CD8⁺CXCR3⁺	848	1208	1105	1308	.12	0.63
CD4⁺CXCR3⁺	22075	53070	29705	35102	.08	0.65

Chemokine	Controls (n = 67/65)*		cGVHD cases (n = 52/44)*		P	ROC AUC
Chemokine	Mean	SD	Mean	SD	P	ROC AUC
Replication set 1
CXCL10, pg/mL	129	105	607	2194	.04	0.61
CXCL9, pg/mL	8969	9218	17715	18675	.02	0.63
Subset (% of parent population)†
CD3^–CD56^brightCXCR3⁺	12.6	10.8	10.3	11.5	.03	0.62
CD8⁺CXCR3⁺	23.4	12.9	22.1	12.8	.50	0.54
CD4⁺CXCR3⁺	13.2	8.5	11.0	8.2	.10	0.59
Ratio CXCL10/%
CD3^–CD56^brightCXCR3⁺	24.3	37.8	159	447	.03	0.63
CD8⁺CXCR3⁺	7.9	9.2	35.2	103.2	.13	0.58
CD4⁺CXCR3⁺	17.9	25.7	154	610	.04	0.61
Ratio CXCL9/%
CD3^–CD56^brightCXCR3⁺	1760	3090	7079	13023	.008	0.65
CD8⁺CXCR3⁺	574	808	1256	1570	.04	0.62
CD4⁺CXCR3⁺	1246	1883	3065	4870	.01	0.64
Replication set 2	(n = 25)		(n = 24)
CXCL10, pg/mL	238.6	449.4	819.5	1370	.005	0.73
CXCL9, pg/mL	16036	25116	25805	31451	.12	0.63
Subset (% of parent population)†
CD3^–CD56^brightCXCR3⁺	16.0	10.62	9.37	5.73	.01	0.70
CD8⁺CXCR3⁺	25.64	15.02	25.39	12.69	.98	0.51
CD4⁺CXCR3⁺	2.38 ± 2.43		1.90	2.32	.38	0.57
Ratio CXCL10/%
CD3^–CD56^brightCXCR3⁺	19.08	31.48	143.0	201.9	.0009	0.78
CD8⁺CXCR3⁺	14.43	33.96	48.6	113.7	.006	0.73
CD4⁺CXCR3⁺	223.3	336.1	1087	2371	.008	0.72
Ratio CXCL9/%
CD3^–CD56^brightCXCR3⁺	1155	1350	5887	11457	.05	0.67
CD8⁺CXCR3⁺	848	1208	1105	1308	.12	0.63
CD4⁺CXCR3⁺	22075	53070	29705	35102	.08	0.65

Number for analysis of CXCL10/CXCL9.

Each CXCR3⁺ subpopulation was collected as a percentage of its parent population of NK, CD4⁺, and CD8⁺ T cells.