Comparison of the experimental designs between Vukovic et al and Tabuko et al
| . | Vukovic et al . | Tabuko et al2 . |
|---|---|---|
| Models . | Hif-1αfl/fl;Mx1-Cre and Hif-1αfl/fl;Vav-iCre . | Hif-1αfl/fl;Mx1-Cre . |
| Assessed HSC function when Hif-1α deletion occurs in both hematopoietic and nonhematopoietic tissues | No | Yes |
| Used serial transplantation assays to evaluate the cell-autonomous role of Hif-1α on HSC self-renewal | Yes | No |
| Used reconstitution assays to evaluate the hematopoietic-specific contribution of Hif-1α | Yes | Yes |
| Time of analysis post Hif-1a deletion | ∼0.5 and 6 months | ∼4 and 11 months |
| Time of analysis posttransplant | ∼2.5 and 8 months | ∼8 and 15 months |
| Impact of Hif-1α deletion on HSC responses to hematopoietic injury | Yes (no observed differences in survival) | Yes (observed differences in survival) |
| Dose of 5-FU | 2 doses of 150 mg/kg | 5-6 doses of 150 mg/kg |
| Time of first administration of 5-FU following post–Hif-1α deletion | Not specified | Not specified |
| Length of assays following Hif-1α deletion | ∼20-30 days | ∼50-80 days |
| Significant difference in survival following serial 5-FU treatment (P value) | No | Yes (P < .001) |
| Conclusion | Cell-intrinsic Hif-1α expression is not required for HSC survival and self-renewal | Hif-1α expression regulates HSC function |
| . | Vukovic et al . | Tabuko et al2 . |
|---|---|---|
| Models . | Hif-1αfl/fl;Mx1-Cre and Hif-1αfl/fl;Vav-iCre . | Hif-1αfl/fl;Mx1-Cre . |
| Assessed HSC function when Hif-1α deletion occurs in both hematopoietic and nonhematopoietic tissues | No | Yes |
| Used serial transplantation assays to evaluate the cell-autonomous role of Hif-1α on HSC self-renewal | Yes | No |
| Used reconstitution assays to evaluate the hematopoietic-specific contribution of Hif-1α | Yes | Yes |
| Time of analysis post Hif-1a deletion | ∼0.5 and 6 months | ∼4 and 11 months |
| Time of analysis posttransplant | ∼2.5 and 8 months | ∼8 and 15 months |
| Impact of Hif-1α deletion on HSC responses to hematopoietic injury | Yes (no observed differences in survival) | Yes (observed differences in survival) |
| Dose of 5-FU | 2 doses of 150 mg/kg | 5-6 doses of 150 mg/kg |
| Time of first administration of 5-FU following post–Hif-1α deletion | Not specified | Not specified |
| Length of assays following Hif-1α deletion | ∼20-30 days | ∼50-80 days |
| Significant difference in survival following serial 5-FU treatment (P value) | No | Yes (P < .001) |
| Conclusion | Cell-intrinsic Hif-1α expression is not required for HSC survival and self-renewal | Hif-1α expression regulates HSC function |
5-FU, 5-flurouracil.