Tm of VWF A2 domain variants as determined by DSF
| . | Tm (°C) . | |
|---|---|---|
| 1 mM EDTA . | 5 mM CaCl2 . | |
| WT | 48.2 ± 0.05 | 64.3 ± 0.13 |
| N1515Q | 48.0 ± 0.04 | 63.1 ± 0.07 |
| N1574Q | 38.2 ± 0.17 | 57.5 ± 0.07 |
| N1515Q/N1574Q | 37.3 ± 0.23 | 57.5 ± 0.05 |
| GnT−/− | 47.5 ± 0.04 | 64.3 ± 0.07 |
| EndoH | 45.5 ± 0.04 | 63.0 ± 0.08 |
| PNGase | 40.4 ± 0.11 | 58.3 ± 0.07 |
| R1575V | 47.6 ± 0.06 | 60.9 ± 0.05 |
| R1575V/N1574Q | 39.6 ± 0.08 | 55.4 ± 0.04 |
| Y1544A | 47.9 ± 0.05 | 63.6 ± 0.15 |
| Y1544A/N1574Q | 43.1 ± 0.07 | 60.8 ± 0.16 |
| Y1544D | 53.5 ± 0.07 | 68.5 ± 0.12 |
| Y1544D/N1574Q | 52.4 ± 0.07 | 67.2 ± 0.10 |
| Y1544R | 45.3 ± 0.16 | 63.1 ± 0.10 |
| N1574Q/Y1544R | 32.3 ± 0.52 | 58.8 ± 0.09 |
| N1515Q/N1574Q/Y1544D | 52.7 ± 0.08 | 67.6 ± 0.32 |
| . | Tm (°C) . | |
|---|---|---|
| 1 mM EDTA . | 5 mM CaCl2 . | |
| WT | 48.2 ± 0.05 | 64.3 ± 0.13 |
| N1515Q | 48.0 ± 0.04 | 63.1 ± 0.07 |
| N1574Q | 38.2 ± 0.17 | 57.5 ± 0.07 |
| N1515Q/N1574Q | 37.3 ± 0.23 | 57.5 ± 0.05 |
| GnT−/− | 47.5 ± 0.04 | 64.3 ± 0.07 |
| EndoH | 45.5 ± 0.04 | 63.0 ± 0.08 |
| PNGase | 40.4 ± 0.11 | 58.3 ± 0.07 |
| R1575V | 47.6 ± 0.06 | 60.9 ± 0.05 |
| R1575V/N1574Q | 39.6 ± 0.08 | 55.4 ± 0.04 |
| Y1544A | 47.9 ± 0.05 | 63.6 ± 0.15 |
| Y1544A/N1574Q | 43.1 ± 0.07 | 60.8 ± 0.16 |
| Y1544D | 53.5 ± 0.07 | 68.5 ± 0.12 |
| Y1544D/N1574Q | 52.4 ± 0.07 | 67.2 ± 0.10 |
| Y1544R | 45.3 ± 0.16 | 63.1 ± 0.10 |
| N1574Q/Y1544R | 32.3 ± 0.52 | 58.8 ± 0.09 |
| N1515Q/N1574Q/Y1544D | 52.7 ± 0.08 | 67.6 ± 0.32 |
Results are means ± SEM of at least 3 independent experiments.