Frequency of Venus+ cells in embryonic tissues of G2V embryos
| Tissue . | Stage . | Number of experiments, embryos analyzed . | % Venus+ cells/tissue . |
|---|---|---|---|
| AGM | E9, 16-25 sp | n = 4, 22 | 1.82 ± 0.31 |
| E10, 28-36 sp | n = 4, 29 | 3.27 ± 0.52 | |
| E11, 43-49 sp | n = 4, 25 | 7.86 ± 1.1 | |
| FL | E9 | nd | nd |
| E10, 28-36 sp | n = 4, 10 | 13.89 ± 0.7 | |
| E11, 43-49 sp | n = 4, 19 | 19.27 ± 2.14 | |
| YS | E9, 16-25 sp | n = 5, 8 | 6.28 ± 0.47 |
| E10, 28-36 sp | n = 6, 9 | 6.17 ± 0.86 | |
| E11, 42-46 sp | n = 1, 3 | 5.25 ± 0.59 | |
| PL | E9, 17-23 sp | n = 3, 4 | 10.91 ± 0.49 |
| E10, 28-35 sp | n = 3, 6 | 10.8 ± 1.92 | |
| E11, 42-46 sp | n = 1, 3 | 10.01 ± 4.64 |
| Tissue . | Stage . | Number of experiments, embryos analyzed . | % Venus+ cells/tissue . |
|---|---|---|---|
| AGM | E9, 16-25 sp | n = 4, 22 | 1.82 ± 0.31 |
| E10, 28-36 sp | n = 4, 29 | 3.27 ± 0.52 | |
| E11, 43-49 sp | n = 4, 25 | 7.86 ± 1.1 | |
| FL | E9 | nd | nd |
| E10, 28-36 sp | n = 4, 10 | 13.89 ± 0.7 | |
| E11, 43-49 sp | n = 4, 19 | 19.27 ± 2.14 | |
| YS | E9, 16-25 sp | n = 5, 8 | 6.28 ± 0.47 |
| E10, 28-36 sp | n = 6, 9 | 6.17 ± 0.86 | |
| E11, 42-46 sp | n = 1, 3 | 5.25 ± 0.59 | |
| PL | E9, 17-23 sp | n = 3, 4 | 10.91 ± 0.49 |
| E10, 28-35 sp | n = 3, 6 | 10.8 ± 1.92 | |
| E11, 42-46 sp | n = 1, 3 | 10.01 ± 4.64 |
The frequency of Venus+ cells within the viable cell fraction of embryonic tissues per embryo is presented. FACS analysis of single cell suspensions of dissected embryonic tissues was performed to define the percentage of cells expressing Venus. AGM contains part of the vitelline and umbilical arteries. Our data for the total number of cells in each tissue (data not shown) correlated with published data for YS and E9.5 AGM29 and for FL.30 The data represent mean ± SEM of 3 to 6 independent experiments, with the exception of E11 YS and PL data (data represent the mean ± standard deviation of 1 experiment. E, embryonic day; n, number of independent experiments, number of individual embryos analyzed; nd, not done; sp, somite pairs.