Effect of fibrin on parameters of factor Xase complex
| . | KM (fX) nM . | VMax (fXa formed) nM/5 min . | KD apparent fVIIIa < − > fIXa nM . | KD apparent (phospholipid*) µM . |
|---|---|---|---|---|
| Control | 57 ± 10 | 2.5 ± 0.1 | 19 ± 2 | 37 ± 4 |
| + fibrin | 32 ± 5 | 3.7 ± 0.2 | 5 ± 1 | 32 ± 6 |
| . | KM (fX) nM . | VMax (fXa formed) nM/5 min . | KD apparent fVIIIa < − > fIXa nM . | KD apparent (phospholipid*) µM . |
|---|---|---|---|---|
| Control | 57 ± 10 | 2.5 ± 0.1 | 19 ± 2 | 37 ± 4 |
| + fibrin | 32 ± 5 | 3.7 ± 0.2 | 5 ± 1 | 32 ± 6 |
Parameters were evaluated in the presence or absence of 20 µg/mL fibrin. Fibrinogen was exposed to 0.1 u/mL thrombin for 2 minutes prior to the addition of 0.3 u/mL hirudin. Concentrations of each component were varied to evaluate the KM, VMax, and apparent dissociation constants. Baseline concentrations were as per Figure 4 description.
Extruded PLVs had composition of PS:PE:PC (4:20:76).