Analysis of erythropoiesis of Tfr2BMKO mice
| Treatment . | Population . | C, % . | Tfr2BMKO, % . | Statistics . |
|---|---|---|---|---|
| IB | Ter119 | 16.08 ± 1.72 | 22.97 ± 1.61 | * |
| I (proerythroblasts) | 0.16 ± 0.02 | 0.29 ± 0.07 | NS | |
| II (basophilic erythroblasts) | 1.14 ± 0.06 | 2.23 ± 0.21 | ** | |
| III (polychromatic erythroblasts) | 2.36 ± 0.12 | 4.43 ± 0.61 | * | |
| IV (orthochromatic erythroblasts) | 12.43 ± 1.53 | 16.03 ± 0.8 | NS | |
| ID | Ter119 | 22.8 ± 1.47 | 18.41 ± 1.57% | NS |
| I (proerythroblasts) | 0.17 ± 0.02 | 0.17 ± 0.03 | NS | |
| II (basophilic erythroblasts) | 2.97 ± 0.36 | 2.37 ± 0.17 | NS | |
| III (polychromatic erythroblasts) | 5.34 ± 0.42 | 4.47 ± 0.28 | NS | |
| IV (orthochromatic erythroblasts) | 14.33 ± 1.14% | 11.41 ± 1.48% | NS |
| Treatment . | Population . | C, % . | Tfr2BMKO, % . | Statistics . |
|---|---|---|---|---|
| IB | Ter119 | 16.08 ± 1.72 | 22.97 ± 1.61 | * |
| I (proerythroblasts) | 0.16 ± 0.02 | 0.29 ± 0.07 | NS | |
| II (basophilic erythroblasts) | 1.14 ± 0.06 | 2.23 ± 0.21 | ** | |
| III (polychromatic erythroblasts) | 2.36 ± 0.12 | 4.43 ± 0.61 | * | |
| IV (orthochromatic erythroblasts) | 12.43 ± 1.53 | 16.03 ± 0.8 | NS | |
| ID | Ter119 | 22.8 ± 1.47 | 18.41 ± 1.57% | NS |
| I (proerythroblasts) | 0.17 ± 0.02 | 0.17 ± 0.03 | NS | |
| II (basophilic erythroblasts) | 2.97 ± 0.36 | 2.37 ± 0.17 | NS | |
| III (polychromatic erythroblasts) | 5.34 ± 0.42 | 4.47 ± 0.28 | NS | |
| IV (orthochromatic erythroblasts) | 14.33 ± 1.14% | 11.41 ± 1.48% | NS |
Mice were analyzed 4 months after transplantation with a WT (controls) or a Tfr2−/− (Tfr2BMKO) BM. Mice were fed as in legend to Figure 1 (see text for details). Viable cells (impermeable to PI) from BM were analyzed for Ter119/CD44 expression. Ter119+ cells were gated and further analyzed with respect of FSC and CD44 surface expression as described in Figure 3A. Table represents percentage of Ter119+ cells on alive cells (RBC not included) and subpopulation composition (gated cluster I-IV). Values are shown as mean ± standard error of the mean (SEM). The number of the events corresponding to the % Ter119+ cells reported (mean ± SEM) is the following: in iron balance 8042 ± 859 for controls and 11 487 ± 806 for Tfr2BMKO; in iron deficiency 11 402 ± 736 for controls and 9204 ± 786 for Tfr2BMKO mice.
NS, not significant.
Asterisks refer to a statistically significant difference. *P < .05; **P < .01