Primer sequences used
| Primer name . | Sequence 5′-3′ . |
|---|---|
| Mpl5-F | GGAAGCTGTCTCGTCTCAGG |
| Mpl5-R | CTAGTGCGGTCTTGTTGCTG |
| Mpl9-F | CCGAGCTCGCTACAGCTT |
| Mpl9-R | CTGTAGTGCGCAGGAAATTG |
| Mpl-TR-F | GAGGACTGGAAGGAGACTGAGGCA |
| Mpl-TR-R | AGGTTGCAGTCCTCTGTAGTCCAT |
| Gapdh-F | GGAGCCAAACGGGTCATCATCTC |
| Gapdh-R | GAGGGGCCATCCACAGTCTTCT |
| Mpl-chip-1F | TTGGTGAAATGGGGAGAAAG |
| Mpl-chip-1R | CTCAGATCCCTGAACCTGGA |
| Mpl-chip-2F | AGCCAGGGCTACACAGAGAA |
| Mpl-chip-2R | AAGCTGTTTGCTTGGGTTGT |
| Mpl-chip-3F | CAGGGCTGAAGCTGGTCTAC |
| Mpl-chip-3R | AGGAAACAAAAAGCATCTGACC |
| Mpl-chip-4F | CTGGACTCCACTCCACACCT |
| Mpl-chip-4R | ATGGCTCATGCCTGTAATCC |
| Mpl-chip-5F | ATGCTGGGGTAACAGGAGTG |
| Mpl-chip-5R | CTGACCACTCTGCCAAGACA |
| Mpl-chip-6F | GCACTGTGGGACAAGAGTCA |
| Mpl-chip-6R | TCCCAACTCAATCCCAAGTC |
| Mpl-chip-7F | ACAGGGCATGGCAACTTTAG |
| Mpl-chip-7R | GATCACTCCAGATGGCTGCT |
| Ott1-HA-F | GGGAATTCCATGTACCCATACGATGTTCCGGATTACGCTATGAGGTCTGCGGGGCGGGAG |
| Ott1-HA-R | TCATCCGCTGTTCACCAGTTTTG |
| Mpl-HA-F | CCGGAATTCGGGAATTCCATGTACCCATACGATGTTCCGGATTA GCTATGCCCTCTTGGGCCCTCTT ATGGTCACTCCTGCCTC |
| Mpl-HA-R | CGGATTCTTAGAAGGACTTAGGGCTGCAGTGTCTCTGAGGTACTGGCCTAGGACCCGGTGTAGGTCTGGAAGCGAGGGCCACAAAGCATGCCTCAGTCTCCTTCCAGTCCTCACGGCCT |
| Mpl-rev | TCAGGGCTGCTGCCAATAGCTTAG T |
| hMPL-TR-F | GAGACCGCCTGGATCTCCTTGGT |
| hMPL-TR-R | TCAGGGCTGCAGTGTCCCTAAG |
| hMPL-9-F | GCGATCTCGCTACCGTTTAC |
| hMPL-9-R | AGGAAACTGCCACCTCAGC |
| hGAPDH-F | AGGGCTGCTTTTAACTCTGGT |
| hGAPDH-R | CCCCACTTGATTTTGGAGGGA |
| Primer name . | Sequence 5′-3′ . |
|---|---|
| Mpl5-F | GGAAGCTGTCTCGTCTCAGG |
| Mpl5-R | CTAGTGCGGTCTTGTTGCTG |
| Mpl9-F | CCGAGCTCGCTACAGCTT |
| Mpl9-R | CTGTAGTGCGCAGGAAATTG |
| Mpl-TR-F | GAGGACTGGAAGGAGACTGAGGCA |
| Mpl-TR-R | AGGTTGCAGTCCTCTGTAGTCCAT |
| Gapdh-F | GGAGCCAAACGGGTCATCATCTC |
| Gapdh-R | GAGGGGCCATCCACAGTCTTCT |
| Mpl-chip-1F | TTGGTGAAATGGGGAGAAAG |
| Mpl-chip-1R | CTCAGATCCCTGAACCTGGA |
| Mpl-chip-2F | AGCCAGGGCTACACAGAGAA |
| Mpl-chip-2R | AAGCTGTTTGCTTGGGTTGT |
| Mpl-chip-3F | CAGGGCTGAAGCTGGTCTAC |
| Mpl-chip-3R | AGGAAACAAAAAGCATCTGACC |
| Mpl-chip-4F | CTGGACTCCACTCCACACCT |
| Mpl-chip-4R | ATGGCTCATGCCTGTAATCC |
| Mpl-chip-5F | ATGCTGGGGTAACAGGAGTG |
| Mpl-chip-5R | CTGACCACTCTGCCAAGACA |
| Mpl-chip-6F | GCACTGTGGGACAAGAGTCA |
| Mpl-chip-6R | TCCCAACTCAATCCCAAGTC |
| Mpl-chip-7F | ACAGGGCATGGCAACTTTAG |
| Mpl-chip-7R | GATCACTCCAGATGGCTGCT |
| Ott1-HA-F | GGGAATTCCATGTACCCATACGATGTTCCGGATTACGCTATGAGGTCTGCGGGGCGGGAG |
| Ott1-HA-R | TCATCCGCTGTTCACCAGTTTTG |
| Mpl-HA-F | CCGGAATTCGGGAATTCCATGTACCCATACGATGTTCCGGATTA GCTATGCCCTCTTGGGCCCTCTT ATGGTCACTCCTGCCTC |
| Mpl-HA-R | CGGATTCTTAGAAGGACTTAGGGCTGCAGTGTCTCTGAGGTACTGGCCTAGGACCCGGTGTAGGTCTGGAAGCGAGGGCCACAAAGCATGCCTCAGTCTCCTTCCAGTCCTCACGGCCT |
| Mpl-rev | TCAGGGCTGCTGCCAATAGCTTAG T |
| hMPL-TR-F | GAGACCGCCTGGATCTCCTTGGT |
| hMPL-TR-R | TCAGGGCTGCAGTGTCCCTAAG |
| hMPL-9-F | GCGATCTCGCTACCGTTTAC |
| hMPL-9-R | AGGAAACTGCCACCTCAGC |
| hGAPDH-F | AGGGCTGCTTTTAACTCTGGT |
| hGAPDH-R | CCCCACTTGATTTTGGAGGGA |