List of genes upregulated in miR-142−/− B cells that have a known role in B cell development and function
| Gene symbol . | Gene title . | Fold change KO vs WT . | P . | B-cell–related process . |
|---|---|---|---|---|
| RAG1 | Recombination activating gene 1 | 18.2635 | .0002 | VDJ recombination pathway ● |
| VPREB1 | Pre–B-lymphocyte gene 1 | 11.2706 | 1.12E−06 | Pre-BCR receptor complex ▪ |
| DNTT | Deoxynucleotidyltransferase, terminal | 4.8675 | 9.4E−05 | VDJ recombination pathway ● |
| AFAP1 | Actin filament–associated protein 1 | 3.6832 | 2.66E−05 | Actin cytoskeleton rearrangement ○ |
| IGLL1 | Immunoglobulin λ-like polypeptide 1 | 3.476 | .0005 | Pre-BCR receptor complex ▪ |
| DSTN | Destrin | 2.9908 | .0003 | Actin cytoskeleton rearrangement ○ |
| MYB | Myeloblastosis oncogene | 2.6885 | .001 | Transcription regulation |
| RHOQ | ras homolog gene family, member Q | 2.4311 | 4.67E−07 | Actin cytoskeleton rearrangement ○ |
| CD93 | CD93 antigen | 2.4244 | .0016 | Cell adhesion |
| RAG2 | Recombination activating gene 2 | 2.3119 | .0003 | VDJ recombination pathway ● |
| CFL2 | Cofilin 2, muscle | 2.2724 | 6.85E−05 | Actin cytoskeleton rearrangement ○ |
| WASL | Wiskott-Aldrich syndrome–like (human) | 1.8902 | 4.63E−05 | Actin cytoskeleton rearrangement ○ |
| TGFBR1 | TGF, β receptor I | 1.6585 | .0008 | TGF-β receptor signaling pathway |
| IL6ST | Interleukin-6 signal transducer | 1.613 | .0002 | IL-6 receptor signaling pathway |
| TNFRSF13c/BAFF-R | TNF receptor superfamily, member13C | 1.4771 | .0002 | BAFF-R signaling pathway ♦ |
| NFKB2 | Nuclear factor of κ light polypeptide gene enhancer in B cells 2, p49/pl00 | 1.3369 | .0033 | BAFF-R signaling pathway ♦ |
| TRAF3 | TNF receptor–associated factor 3 | 1.2257 | .0096 | BAFF-R signaling pathway ♦ |
| Gene symbol . | Gene title . | Fold change KO vs WT . | P . | B-cell–related process . |
|---|---|---|---|---|
| RAG1 | Recombination activating gene 1 | 18.2635 | .0002 | VDJ recombination pathway ● |
| VPREB1 | Pre–B-lymphocyte gene 1 | 11.2706 | 1.12E−06 | Pre-BCR receptor complex ▪ |
| DNTT | Deoxynucleotidyltransferase, terminal | 4.8675 | 9.4E−05 | VDJ recombination pathway ● |
| AFAP1 | Actin filament–associated protein 1 | 3.6832 | 2.66E−05 | Actin cytoskeleton rearrangement ○ |
| IGLL1 | Immunoglobulin λ-like polypeptide 1 | 3.476 | .0005 | Pre-BCR receptor complex ▪ |
| DSTN | Destrin | 2.9908 | .0003 | Actin cytoskeleton rearrangement ○ |
| MYB | Myeloblastosis oncogene | 2.6885 | .001 | Transcription regulation |
| RHOQ | ras homolog gene family, member Q | 2.4311 | 4.67E−07 | Actin cytoskeleton rearrangement ○ |
| CD93 | CD93 antigen | 2.4244 | .0016 | Cell adhesion |
| RAG2 | Recombination activating gene 2 | 2.3119 | .0003 | VDJ recombination pathway ● |
| CFL2 | Cofilin 2, muscle | 2.2724 | 6.85E−05 | Actin cytoskeleton rearrangement ○ |
| WASL | Wiskott-Aldrich syndrome–like (human) | 1.8902 | 4.63E−05 | Actin cytoskeleton rearrangement ○ |
| TGFBR1 | TGF, β receptor I | 1.6585 | .0008 | TGF-β receptor signaling pathway |
| IL6ST | Interleukin-6 signal transducer | 1.613 | .0002 | IL-6 receptor signaling pathway |
| TNFRSF13c/BAFF-R | TNF receptor superfamily, member13C | 1.4771 | .0002 | BAFF-R signaling pathway ♦ |
| NFKB2 | Nuclear factor of κ light polypeptide gene enhancer in B cells 2, p49/pl00 | 1.3369 | .0033 | BAFF-R signaling pathway ♦ |
| TRAF3 | TNF receptor–associated factor 3 | 1.2257 | .0096 | BAFF-R signaling pathway ♦ |
Analysis of gene expression in WT (n = 3) and KO (n = 3) CD19+ B cells was performed using DNA microarrays. miR-142-3p putative target genes are indicated in bold.
TGF, transforming growth factor; TNF, tumor necrosis factor.