Cellular content in lymph nodes from leukemic mice
| Lymph node . | Percentage of live cells . | Percentage of CD19+B220+ . | ||||
|---|---|---|---|---|---|---|
| Gr1/Mac1+CD3− . | Gr1/Mac1−CD3+ . | CD19+ B220+ . | CD43highIgM− . | CD43low/negIgM− . | CD43low/negIgM+ . | |
| 277 (Pax5+/−Ebf1+/−) | 0.5 | 0.3 | 90.9 | 77.7 | 17.9 | 0.1 |
| 278 (Pax5+/−Ebf1+/−) | 0.5 | 0.3 | 98.2 | 93.9 | 4.5 | 0.0 |
| 279 (Pax5+/−Ebf1+/−) | 4.1 | 25.6 | 56.7 | 75.1 | 5.4 | 15.4 |
| 292 (Pax5+/−Ebf1+/−) | 1.6 | 1.2 | 95.2 | 58.6 | 36.0 | 0.9 |
| 317 (Pax5+/−Ebf1+/−) | 1.8 | 0.6 | 96.1 | 91.3 | 6.9 | 0.3 |
| 327 (Pax5+/−Ebf1+/−) | 1.1 | 0.5 | 97.4 | 95.0 | 3.3 | 0.2 |
| 381 (Pax5+/−Ebf1+/−) | 0.8 | 4.6 | 90.4 | 78.1 | 7.6 | 4.6 |
| 383 (Pax5+/−Ebf1+/−) | 2.6 | 6.0 | 88.0 | 85.6 | 3.9 | 6.1 |
| 371 (Ebf1+/−) | 3.7 | 4.7 | 81.3 | 82.5 | 10.0 | 3.2 |
| Mean | 1.9 | 4.9 | 88.2 | 82.0 | 10.6 | 3.4 |
| SD | 1.3 | 8.l | 13.0 | 11.4 | 10.5 | 5.0 |
| Lymph node . | Percentage of live cells . | Percentage of CD19+B220+ . | ||||
|---|---|---|---|---|---|---|
| Gr1/Mac1+CD3− . | Gr1/Mac1−CD3+ . | CD19+ B220+ . | CD43highIgM− . | CD43low/negIgM− . | CD43low/negIgM+ . | |
| 277 (Pax5+/−Ebf1+/−) | 0.5 | 0.3 | 90.9 | 77.7 | 17.9 | 0.1 |
| 278 (Pax5+/−Ebf1+/−) | 0.5 | 0.3 | 98.2 | 93.9 | 4.5 | 0.0 |
| 279 (Pax5+/−Ebf1+/−) | 4.1 | 25.6 | 56.7 | 75.1 | 5.4 | 15.4 |
| 292 (Pax5+/−Ebf1+/−) | 1.6 | 1.2 | 95.2 | 58.6 | 36.0 | 0.9 |
| 317 (Pax5+/−Ebf1+/−) | 1.8 | 0.6 | 96.1 | 91.3 | 6.9 | 0.3 |
| 327 (Pax5+/−Ebf1+/−) | 1.1 | 0.5 | 97.4 | 95.0 | 3.3 | 0.2 |
| 381 (Pax5+/−Ebf1+/−) | 0.8 | 4.6 | 90.4 | 78.1 | 7.6 | 4.6 |
| 383 (Pax5+/−Ebf1+/−) | 2.6 | 6.0 | 88.0 | 85.6 | 3.9 | 6.1 |
| 371 (Ebf1+/−) | 3.7 | 4.7 | 81.3 | 82.5 | 10.0 | 3.2 |
| Mean | 1.9 | 4.9 | 88.2 | 82.0 | 10.6 | 3.4 |
| SD | 1.3 | 8.l | 13.0 | 11.4 | 10.5 | 5.0 |
Cellular composition of frozen lymph node samples from mice diagnosed with lymph node enlargement. The number in the far left column indicate the identity of the mice. The FACS analysis was based on surface expression of the myeloid markers Gr1/Mac1, the T-lineage marker CD3, and the B-lineage markers CD19 and B220. The subcomposition of the CD19+B220+ population was further investigated based on the expression of CD43 and IgM. The first 3 columns are percentage of live cells, whereas the last three columns display percentage of CD19+B220+ cells.