Characteristics of mice diagnosed with AML and MDS
| Mouse . | Survival* (d) . | Necropsy findings . | WBC† (×103/μL) . | RBC† (×106/μL) . | PLT† (×103/μL) . | BM histology‡ . | Infiltration§ . | Diagnosis . |
|---|---|---|---|---|---|---|---|---|
| 226 | 139 | HSM, hem | 26.16 | 8.37 | 244 | Blasts | Spleen, liver | AML |
| 229 | 362 | SM, mottled | 19.92 | 8.07 | 1192 | Blasts | Spleen, liver | AML |
| 230 | 221 | Pale, HM | 3.82 | 6.72 | 325 | Blasts | Spleen, liver | AML |
| 231 | 347 | Pale, HM, hem, MF‖ | ND | ND | ND | Blasts | Spleen, liver, kidney | AML |
| 235 | 330 | HSM, pale, hem, MF | 3.08 | 2.69 | 126 | Blasts | Spleen, liver | AML |
| 246 | 326 | SM, hem, MF | ND | ND | ND | Blasts | Spleen, liver | AML |
| 257 | 328 | HM, hem | 0.46 | 6.61 | 556 | Blasts | Spleen, liver | AML |
| 263 | 263 | HSM, mottled, hem | 17.36 | 5.17 | 807 | Blasts | Spleen, liver | AML |
| 223 | 299 | HSM, hem | 4.04 | 4.15 | 424 | Dysplasia | NA | MDS |
| 227 | 358 | HSM, mottled, hem | ND | ND | ND | Dysplasia | NA | MDS |
| 232 | 355 | SM, hem, pale, MF | ND | ND | ND | Dysplasia | NA | MDS |
| 236 | 295 | HSM | 8.42 | 4.34 | 859 | Dysplasia | NA | MDS |
| 237 | 315 | HM, mottled, hem, pale | ND | ND | ND | Dysplasia | NA | MDS |
| 238 | 196 | SM, hem | 1.28 | 2.54 | 142 | Dysplasia | NA | MDS |
| 242 | 321 | HSM, mottled, hem | 2.44 | 1.49 | 146 | Dysplasia | NA | MDS |
| 252 | 319 | SM, hem, MF | 19.08 | 8.33 | 353 | Dysplasia | NA | MDS |
| 256 | 326 | Dark SP, hem | 4.1 | 6.18 | 556 | Dysplasia | NA | MDS |
| 258 | 316 | HM, hem | ND | ND | ND | Dysplasia | NA | MDS |
| Mouse . | Survival* (d) . | Necropsy findings . | WBC† (×103/μL) . | RBC† (×106/μL) . | PLT† (×103/μL) . | BM histology‡ . | Infiltration§ . | Diagnosis . |
|---|---|---|---|---|---|---|---|---|
| 226 | 139 | HSM, hem | 26.16 | 8.37 | 244 | Blasts | Spleen, liver | AML |
| 229 | 362 | SM, mottled | 19.92 | 8.07 | 1192 | Blasts | Spleen, liver | AML |
| 230 | 221 | Pale, HM | 3.82 | 6.72 | 325 | Blasts | Spleen, liver | AML |
| 231 | 347 | Pale, HM, hem, MF‖ | ND | ND | ND | Blasts | Spleen, liver, kidney | AML |
| 235 | 330 | HSM, pale, hem, MF | 3.08 | 2.69 | 126 | Blasts | Spleen, liver | AML |
| 246 | 326 | SM, hem, MF | ND | ND | ND | Blasts | Spleen, liver | AML |
| 257 | 328 | HM, hem | 0.46 | 6.61 | 556 | Blasts | Spleen, liver | AML |
| 263 | 263 | HSM, mottled, hem | 17.36 | 5.17 | 807 | Blasts | Spleen, liver | AML |
| 223 | 299 | HSM, hem | 4.04 | 4.15 | 424 | Dysplasia | NA | MDS |
| 227 | 358 | HSM, mottled, hem | ND | ND | ND | Dysplasia | NA | MDS |
| 232 | 355 | SM, hem, pale, MF | ND | ND | ND | Dysplasia | NA | MDS |
| 236 | 295 | HSM | 8.42 | 4.34 | 859 | Dysplasia | NA | MDS |
| 237 | 315 | HM, mottled, hem, pale | ND | ND | ND | Dysplasia | NA | MDS |
| 238 | 196 | SM, hem | 1.28 | 2.54 | 142 | Dysplasia | NA | MDS |
| 242 | 321 | HSM, mottled, hem | 2.44 | 1.49 | 146 | Dysplasia | NA | MDS |
| 252 | 319 | SM, hem, MF | 19.08 | 8.33 | 353 | Dysplasia | NA | MDS |
| 256 | 326 | Dark SP, hem | 4.1 | 6.18 | 556 | Dysplasia | NA | MDS |
| 258 | 316 | HM, hem | ND | ND | ND | Dysplasia | NA | MDS |
BM, bone marrow; dark Sp, dark spleen color; hem, hemorrhaging; HM, hepatomegaly; HSM, hepatosplenomegaly; MF, myelofibrosis; mottled, mottled liver; pale, pale paw pads and/or ears; PLT, platelet; RBC, red blood cell; SM, splenomegaly.
Survival indicates time from transplant to natural death or induced death if moribund.
WBC, RBC, and PLT counts are indicated for mice that had a CBC done at the time of death. Control ranges: WBC, 16.7-19.7 × 103/μL; RBC, 9.08-9.86 × 106/μL; PLT, 656-976 × 103/μL. ND indicates that a CBC was not done at the time of death. In these cases, blood smears and CBCs from previous time points were indicative of anemia and/or thrombocytopenia.
“Blasts” indicates >20% blasts in BM; “dysplasia” indicates that dysplasia was observed in 1 or more lineages (see also supplemental Figure 2).
Infiltration of indicated tissues was determined by histology and/or flow cytometry. “Spleen” indicates an increase specifically in nonlymphoid cells. NA indicates that this phenotype is not applicable to MDS.
MF determined by positive reticulin staining posttransplant.