Regulators of HSC quiescence
| Gene symbol . | Description . | Fold-change untreated . | P value untreated . | Regulation untreated . | Fold-change poly:IC . | P value poly:IC . | Regulation poly:IC . |
|---|---|---|---|---|---|---|---|
| Egr1 | Early growth response 1 | 2.90 | 2.11E-03 | Up | 2.62 | 1.25E-02 | Up |
| Atm | Ataxia telangiectasia mutated homolog (human) | 1.50 | 6.02E-01 | — | 2.08 | 4.62E-02 | Down |
| Nurr1 | Nuclear receptor subfamily 4, group A, member 2, transcript variant 1 | 4.03 | 1.01E-03 | Up | 2.68 | 7.45E-02 | — |
| Cdkn2c | CDK inhibitor 2C (p18, inhibits CDK4) | 2.21 | 2.36E-01 | — | 2.16 | 1.71E-01 | — |
| Gfi1 | Growth factor independent 1 | 1.12 | 6.56E-01 | — | 1.96 | 2.77E-02 | — |
| Stat1 | Signal transducer and activator of transcription 1, transcript variant 2 | 1.07 | 6.48E-01 | — | 1.75 | 2.24E-02 | — |
| Hoxb9 | Homeobox B9 | 1.27 | 4.77E-01 | — | 1.70 | 4,52E-02 | — |
| Junb | Jun B proto-oncogene | 1.21 | 2.92E-01 | — | 1.65 | 8,99E-02 | — |
| Pml | Promyelocytic leukemia, transcript variant 2 | 1.22 | 8.64E-02 | — | 1.64 | 4.62E-02 | — |
| Foxo4 | Forkhead box O4 | 1.75 | 1.89E-03 | — | 1.54 | 2.93E-01 | — |
| Hoxa4 | Homeobox A4 | 1.03 | 8.27E-01 | — | 1.47 | 3.07E-01 | — |
| Bmi1 | Bmi1 polycomb ring finger oncogene | 1.21 | 1.38E-01 | — | 1.38 | 2.76E-01 | — |
| Trp53 | Transformation related protein 53 | 1.52 | 1.81E-02 | — | 1.35 | 1.26E-01 | — |
| Txnip | Thioredoxin interacting protein | 1.75 | 3.40E-02 | — | 1.27 | 2.42E-01 | — |
| Shh | Sonic hedgehog | 1.25 | 2.30E-02 | — | 1.26 | 2.44E-01 | — |
| Tal1 | T-cell acute lymphocytic leukemia 1, transcript variant 2 | 1.07 | 6.91E-01 | — | 1.25 | 1.93E-01 | — |
| Satb1 | Special AT-rich sequence binding protein 1, transcript variant 2 | 1.65 | 1.71E-01 | — | 1.22 | 4.03E-01 | — |
| Hoxc4 | Homeobox C4 | 6.78 | 8.87E-03 | Up | 1.19 | 7.98E-01 | — |
| Itch | Itchy, E3 ubiquitin protein ligase, transcript variant 2 | 1.21 | 2.00E-01 | — | 1.14 | 7.27E-01 | — |
| Pbx1 | Pre B-cell leukemia homeobox 1 (Pbx1), transcript variant a | 1.27 | 7.09E-02 | — | 1.12 | 3.57E-01 | — |
| Gene symbol . | Description . | Fold-change untreated . | P value untreated . | Regulation untreated . | Fold-change poly:IC . | P value poly:IC . | Regulation poly:IC . |
|---|---|---|---|---|---|---|---|
| Egr1 | Early growth response 1 | 2.90 | 2.11E-03 | Up | 2.62 | 1.25E-02 | Up |
| Atm | Ataxia telangiectasia mutated homolog (human) | 1.50 | 6.02E-01 | — | 2.08 | 4.62E-02 | Down |
| Nurr1 | Nuclear receptor subfamily 4, group A, member 2, transcript variant 1 | 4.03 | 1.01E-03 | Up | 2.68 | 7.45E-02 | — |
| Cdkn2c | CDK inhibitor 2C (p18, inhibits CDK4) | 2.21 | 2.36E-01 | — | 2.16 | 1.71E-01 | — |
| Gfi1 | Growth factor independent 1 | 1.12 | 6.56E-01 | — | 1.96 | 2.77E-02 | — |
| Stat1 | Signal transducer and activator of transcription 1, transcript variant 2 | 1.07 | 6.48E-01 | — | 1.75 | 2.24E-02 | — |
| Hoxb9 | Homeobox B9 | 1.27 | 4.77E-01 | — | 1.70 | 4,52E-02 | — |
| Junb | Jun B proto-oncogene | 1.21 | 2.92E-01 | — | 1.65 | 8,99E-02 | — |
| Pml | Promyelocytic leukemia, transcript variant 2 | 1.22 | 8.64E-02 | — | 1.64 | 4.62E-02 | — |
| Foxo4 | Forkhead box O4 | 1.75 | 1.89E-03 | — | 1.54 | 2.93E-01 | — |
| Hoxa4 | Homeobox A4 | 1.03 | 8.27E-01 | — | 1.47 | 3.07E-01 | — |
| Bmi1 | Bmi1 polycomb ring finger oncogene | 1.21 | 1.38E-01 | — | 1.38 | 2.76E-01 | — |
| Trp53 | Transformation related protein 53 | 1.52 | 1.81E-02 | — | 1.35 | 1.26E-01 | — |
| Txnip | Thioredoxin interacting protein | 1.75 | 3.40E-02 | — | 1.27 | 2.42E-01 | — |
| Shh | Sonic hedgehog | 1.25 | 2.30E-02 | — | 1.26 | 2.44E-01 | — |
| Tal1 | T-cell acute lymphocytic leukemia 1, transcript variant 2 | 1.07 | 6.91E-01 | — | 1.25 | 1.93E-01 | — |
| Satb1 | Special AT-rich sequence binding protein 1, transcript variant 2 | 1.65 | 1.71E-01 | — | 1.22 | 4.03E-01 | — |
| Hoxc4 | Homeobox C4 | 6.78 | 8.87E-03 | Up | 1.19 | 7.98E-01 | — |
| Itch | Itchy, E3 ubiquitin protein ligase, transcript variant 2 | 1.21 | 2.00E-01 | — | 1.14 | 7.27E-01 | — |
| Pbx1 | Pre B-cell leukemia homeobox 1 (Pbx1), transcript variant a | 1.27 | 7.09E-02 | — | 1.12 | 3.57E-01 | — |
Summary of HSC quiescence regulating genes that are differently regulated in Cdk6−/− fraction A cells (listed in order of “fold-change”) in steady state (“untreated”) or upon in vivo poly(I:C) stimulation. In the absence of CDK6, Egr1 levels are significantly higher compared with control. This difference is even more prominent in the poly(I:C)-treated fraction, in which Egr1 represents the most deregulated factor. Statistical significance (fold-change >2; P < .05).