Thermodynamic constants of interaction of msEPCR with mFVIIa or its chimeras using isothermal titration calorimetry
| Sample . | Kd (µM) . | n . | ΔG (kcal/mol) . | ΔH (kcal/mol) . | ΔS (cal/mol/K) . |
|---|---|---|---|---|---|
| mFVIIa | >8 | ND | ND | ND | ND |
| mFVIIa-FMR | 0.48 ± 0.02 | 0.89 | −8.6 | −4.7 ± 0.03 | 13.3 |
| mFVIIa-(1-43 mPC) | 0.11 ± 0.01 | 0.85 | −9.5 | −4.6 ± 0.03 | 16.4 |
| Sample . | Kd (µM) . | n . | ΔG (kcal/mol) . | ΔH (kcal/mol) . | ΔS (cal/mol/K) . |
|---|---|---|---|---|---|
| mFVIIa | >8 | ND | ND | ND | ND |
| mFVIIa-FMR | 0.48 ± 0.02 | 0.89 | −8.6 | −4.7 ± 0.03 | 13.3 |
| mFVIIa-(1-43 mPC) | 0.11 ± 0.01 | 0.85 | −9.5 | −4.6 ± 0.03 | 16.4 |
All parameters are listed as ±67% confidence intervals. Kd, equilibrium dissociation constant; n, stoichiometry of interaction; ND, not determined; ΔG, change in Gibbs free energy (G); ΔH, change in enthalpy (H); ΔS, change in entropy (S).