Summary of IC injections
| Mother . | Absolute cell no. per pup . | No. cells/kg per pup . | CD34 cells/kg . | CD3 cells/kg . | Cell selection . | GA . | No. injected . | Injected survival to birth . |
|---|---|---|---|---|---|---|---|---|
| Betty | 1.3 × 108 | 2.5 × 1010 | 7.7 × 108 | 2.5 × 108 | CD3 depletion | 39 | (6/6) | 6 |
| Minnow | 3.5 × 108 | 5.2 × 1010 | 1.7 × 109 | 3.7 × 108 | CD3 depletion | 40 | (4/4) | 3 |
| Voodoo | 3.1 × 108 | 4.0 × 1010 | 1.1 × 109 | 4.1 × 108 | CD3 depletion | 40.5 | (5/5) | 4 |
| Vinnie | 3.5 × 108 | 4.6 × 1010 | 9.1 × 108 | 4.0 × 108 | CD3 depletion | 40.5 | (6/6) | 6 |
| Julia | 2.3 × 108 | 2.5 × 1010 | 5.7 × 108 | 4.0 × 108 | CD3 depletion | 41 | (6/6) | 4 |
| Bella* | 1.8 × 108 | 1.6 × 1010 | 1.7 × 109 | 3.9 × 108 | CD34 selection | 42.5 | (2/4) | 2 |
| Mother . | Absolute cell no. per pup . | No. cells/kg per pup . | CD34 cells/kg . | CD3 cells/kg . | Cell selection . | GA . | No. injected . | Injected survival to birth . |
|---|---|---|---|---|---|---|---|---|
| Betty | 1.3 × 108 | 2.5 × 1010 | 7.7 × 108 | 2.5 × 108 | CD3 depletion | 39 | (6/6) | 6 |
| Minnow | 3.5 × 108 | 5.2 × 1010 | 1.7 × 109 | 3.7 × 108 | CD3 depletion | 40 | (4/4) | 3 |
| Voodoo | 3.1 × 108 | 4.0 × 1010 | 1.1 × 109 | 4.1 × 108 | CD3 depletion | 40.5 | (5/5) | 4 |
| Vinnie | 3.5 × 108 | 4.6 × 1010 | 9.1 × 108 | 4.0 × 108 | CD3 depletion | 40.5 | (6/6) | 6 |
| Julia | 2.3 × 108 | 2.5 × 1010 | 5.7 × 108 | 4.0 × 108 | CD3 depletion | 41 | (6/6) | 4 |
| Bella* | 1.8 × 108 | 1.6 × 1010 | 1.7 × 109 | 3.9 × 108 | CD34 selection | 42.5 | (2/4) | 2 |
All fetuses received donor inoculum containing 1% CD3, with CD3/kg doses ranging from 2.5 × 108 to 4.1 × 108 cells per kg. CD34 doses varied based on the yield of the maternal BM harvest and ranged from 5.7 × 108 to 1.7 × 109. Fetal weights were calculated using crown-rump measurements according to published normograms for beagle gestation.12
This row marks a litter in which insufficient donor cells were available to inject all fetuses.