Summary of regulated kinases
| Accession . | Gene . | Site . | Regulation . |
|---|---|---|---|
| Kinase early responders, 10 s | |||
| Q8TD19 | NEK9 | Ser827 | ↓105nM |
| Q9UKE5 | TNIK | Ser1021/1028 | ↑105nM |
| Ser764/Ser766/Ser769 | ↑105nM | ||
| P07948 | LYN | Thr489/Tyr492/Ser495/Tyr501/Thr502/Thr504 | ↑60/302/5nM |
| Q00537 | CDK17 | Ser9 | ↑105nM |
| Ser180 | ↑105nM | ||
| Ser182 | ↑102nM | ||
| Q02750 | MAP2K1 | Ser377/Thr378/Ser385/Thr386/Thr388 | ↓105nM |
| Ser385/Thr386 | ↑105nM | ||
| Q93100 | PHKB | Ser27 | ↑102/5nM |
| Ser29 | ↑102/5nM | ||
| Q8N4C8 | MNK1 | Ser699 | ↑102/5nM |
| Ser701 | ↑102/5nM | ||
| Ser732 | ↓105nM | ||
| Ser993 | ↑105nM | ||
| Q9BZ23 | PANK2 | Ser189 | ↑30/102/5nM |
| O95819 | MAP4K4 | Ser900/Thr907/Thr909/Ser913 | ↑102/5nM |
| Q15746 | MLCK | Ser1438 | ↓105nM |
| Ser1773 | ↑30/102/5nM | ||
| P12931 | SRC | Tyr530 | ↑105nM |
| Kinase late responders, >10 s | |||
| Q12851 | MAP4K2 | Ser365/367 | ↑305nM |
| P46019 | PHKA2 | Ser729 | ↑302/5nM |
| P52333 | JAK3 | Ser17 | ↑605nM |
| P49840 | GSK3A | Thr19/Ser20/Ser21/Ser39 | ↑105nM |
| P27448 | MARK3 | Tyr441/Ser442 | ↑305nM |
| Accession . | Gene . | Site . | Regulation . |
|---|---|---|---|
| Kinase early responders, 10 s | |||
| Q8TD19 | NEK9 | Ser827 | ↓105nM |
| Q9UKE5 | TNIK | Ser1021/1028 | ↑105nM |
| Ser764/Ser766/Ser769 | ↑105nM | ||
| P07948 | LYN | Thr489/Tyr492/Ser495/Tyr501/Thr502/Thr504 | ↑60/302/5nM |
| Q00537 | CDK17 | Ser9 | ↑105nM |
| Ser180 | ↑105nM | ||
| Ser182 | ↑102nM | ||
| Q02750 | MAP2K1 | Ser377/Thr378/Ser385/Thr386/Thr388 | ↓105nM |
| Ser385/Thr386 | ↑105nM | ||
| Q93100 | PHKB | Ser27 | ↑102/5nM |
| Ser29 | ↑102/5nM | ||
| Q8N4C8 | MNK1 | Ser699 | ↑102/5nM |
| Ser701 | ↑102/5nM | ||
| Ser732 | ↓105nM | ||
| Ser993 | ↑105nM | ||
| Q9BZ23 | PANK2 | Ser189 | ↑30/102/5nM |
| O95819 | MAP4K4 | Ser900/Thr907/Thr909/Ser913 | ↑102/5nM |
| Q15746 | MLCK | Ser1438 | ↓105nM |
| Ser1773 | ↑30/102/5nM | ||
| P12931 | SRC | Tyr530 | ↑105nM |
| Kinase late responders, >10 s | |||
| Q12851 | MAP4K2 | Ser365/367 | ↑305nM |
| P46019 | PHKA2 | Ser729 | ↑302/5nM |
| P52333 | JAK3 | Ser17 | ↑605nM |
| P49840 | GSK3A | Thr19/Ser20/Ser21/Ser39 | ↑105nM |
| P27448 | MARK3 | Tyr441/Ser442 | ↑305nM |
Kinases showing an early and late response upon iloprost stimulation. ↓105nM corresponds to downregulation of phosphorylation after 10 seconds in the 5nM iloprost samples. If the phosphorylation site localization was not confident (eg, Lyn, MAPKK1, MEKKK4, and GSK3α), all potentially phosphorylated residues are listed according to phosphoRS.