Decreased Kd values for anti-TNP low-fucose antibodies compared with anti-TNP high-fucose antibodies with respect to FcγRIIIa and FcγRIIIb, but not for FcγRIIa
| . | FcγRIIIa (Kd, µM) ± SD . | FcγRIIIb (Kd, µM) ± SD . | FcγRIIa (Kd, µM) ± SD . |
|---|---|---|---|
| Anti-TNP high fucose | 0.12 ± 0.01 | 0.58 ± 0.11 | 0.22 ± 0.11 |
| Anti-TNP low fucose | 0.05 ± 0.02 | 0.08 ± 0.00 | 0.20 ± 0.08 |
| Paired Student t test anti-TNP high fucose vs anti-TNP low fucose | 0.001, *** | 0.008, ** | 0.2252, NS |
| . | FcγRIIIa (Kd, µM) ± SD . | FcγRIIIb (Kd, µM) ± SD . | FcγRIIa (Kd, µM) ± SD . |
|---|---|---|---|
| Anti-TNP high fucose | 0.12 ± 0.01 | 0.58 ± 0.11 | 0.22 ± 0.11 |
| Anti-TNP low fucose | 0.05 ± 0.02 | 0.08 ± 0.00 | 0.20 ± 0.08 |
| Paired Student t test anti-TNP high fucose vs anti-TNP low fucose | 0.001, *** | 0.008, ** | 0.2252, NS |
SPR analyses of anti-TNP antibodies with high fucose vs anti-TNP antibodies with low fucose, interacting with FcγRIIIa (n = 3), FcγRIIIb (n = 3), and FcγRIIa (n = 4). Kd values (µM): the constant rate of dissociation of antibody from the receptors at equilibrium state, with standard deviation (SD) are shown. One-tailed paired-test outcome is depicted. **P ≤ .01; ***P ≤ 001. NS, nonsignificant.