Table 1 WBC, Plt, Hct, IgG, IgM, HLA I... | American Society of Hematology

Table 1

WBC, Plt, Hct, IgG, IgM, HLA I and II, 2,3-DPG, ATP, and pH in RBC units that underwent experimental filtration or standard LR

Test	Filtration and storage day		Filters
Test	Filtration and storage day		B (female = 11, male = 4)	P (female = 10, male = 4)	M (female = 10, male = 4)	Standard LR, BPF4 (female = 2, male = 2)
WBC (10³/μL)	Pre		6.73 ± 0.54	8.32 ± 0.68	8.07 ± 0.74	9.05 ± 2.05
	Post		0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00
	D21		0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00
	D42		0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00
Plt (10³/μL)	Pre		218.38 ± 26.70	196.33 ± 24.54	258.93 ± 18.09	303.75 ± 87.75
	Post		0.76 ± 0.20	6.13 ± 1.52	0.43 ± 0.24	1.00 ± 0.00
	D21		0.52 ± 0.16	1.00 ± 0.32	4.10 ± 2.35	0.50 ± 0.00
	D42		3.68 ± 2.55	1.30 ± 0.51	1.45 ± 0.43	1.00 ± 0.00
Hct (%)	Pre		59.30 ± 0.63	59.15 ± 0.91	59.24 ± 1.00	60.13 ± 0.78
	Post		55.58 ± 0.96	55.56 ± 0.87	53.04 ± 0.58	60.50 ± 0.50
	D21		51.48 ± 5.78	54.94 ± 0.57	54.70 ± 0.62	63.98 ± 1.68
	D42		57.31 ± 0.85	55.29 ± 0.91	55.32 ± 0.93	62.43 ± 0.18
Hb (g/dL)	Pre		19.8 ± 0.19	19.77 ± 0.21	19.61 ± 0.24	20.3 ± 0.47
	Post		18.56 ± 0.22	18.28 ± 0.25	17.42 ± 0.21	20.35 ± 0.31
	D21		17.11 ± 1.92	18.14 ± 0.22	18.18 ± 0.30	21.50 ± 0.65
	D42		18.89 ± 0.30	18.03 ± 0.43	18.03 ± 0.33	20.60 ± 0.15
IgG (mg/dL)	Pre		121.9 ± 8.1	167.1 ± 31.5	135.3 ± 10.7	110.9 ± 20.5
IgG (mg/dL)	Post		12.4 ± 11.9*	2.64 ± 2.3*	2.23 ± 0.9*	100.5 ± 15.6
IgM (mg/dL)	Pre		8.3 ± 1.1	8.69 ± 1.2	9.4 ± 1.2	10.0 ± 3.7
IgM (mg/dL)	Post		1.3 ± 0.6*	1.1 ± 0.5*	1.9 ± 0.5*	11.9 ± 3.8
MFI	HLA I	Pre	5568.06 ± 938.78	3037.52 ± 202.22	2682.01 ± 178.84	N/A
	HLA I	Post	<2000	<2000	<2000	N/A
	HLA II	Pre	2837.67 ± 163.46	3163.15 ± 202.09	4339.25 ± 583.68	N/A
	HLA II	Post	<2000	<2000	<2000	N/A
HLA I (# of donors)	Pre		2	2	4†	0
HLA I (# of donors)	Post		0	0	0	0
HLA II (# of donors)	Pre		2	2	5†	0
HLA II (# of donors)	Post		0	0	0	0
2,3-DPG (µmol/gHb)	Pre		253.8 ± 12.4	275.0 ± 12.8	259.6 ± 24.2	276.5 ± 12.7
	Post		221.9 ± 6.0	229.2 ± 8.3	209.6 ± 10.0	248.3 ± 10.2
	D21		28.7 ± 3.8	35.7 ± 5.3	27.7 ± 5.2	25.45 ± 3.5
	D42		14.4 ± 1.0*	13.9 ± 1.6*	13.0 ± 2.3*	12.9 ± 0.9*
ATP (µmol/gHb)	Pre		4.4 ± 0.3	4.8 ± 0.1	4.8 ± 0.1	4.4 ± 0.3
	Post		5.1 ± 0.1	5.2 ± 0.2	5.2 ± 0.1	4.5 ± 0.3
	D21		4.7 ± 0.1	4.5 ± 0.2	4.8 ± 0.1	4.4 ± 0.3
	D42		3.1 ± 0.1*	3.1 ± 0.2*	3.3 ± 0.2*	3.3 ± 0.2
pH range (D42/D0)			6.5/7.3	6.6/7.4	6.4/7.3	6.7/7.3

Test	Filtration and storage day		Filters
Test	Filtration and storage day		B (female = 11, male = 4)	P (female = 10, male = 4)	M (female = 10, male = 4)	Standard LR, BPF4 (female = 2, male = 2)
WBC (10³/μL)	Pre		6.73 ± 0.54	8.32 ± 0.68	8.07 ± 0.74	9.05 ± 2.05
	Post		0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00
	D21		0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00
	D42		0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00
Plt (10³/μL)	Pre		218.38 ± 26.70	196.33 ± 24.54	258.93 ± 18.09	303.75 ± 87.75
	Post		0.76 ± 0.20	6.13 ± 1.52	0.43 ± 0.24	1.00 ± 0.00
	D21		0.52 ± 0.16	1.00 ± 0.32	4.10 ± 2.35	0.50 ± 0.00
	D42		3.68 ± 2.55	1.30 ± 0.51	1.45 ± 0.43	1.00 ± 0.00
Hct (%)	Pre		59.30 ± 0.63	59.15 ± 0.91	59.24 ± 1.00	60.13 ± 0.78
	Post		55.58 ± 0.96	55.56 ± 0.87	53.04 ± 0.58	60.50 ± 0.50
	D21		51.48 ± 5.78	54.94 ± 0.57	54.70 ± 0.62	63.98 ± 1.68
	D42		57.31 ± 0.85	55.29 ± 0.91	55.32 ± 0.93	62.43 ± 0.18
Hb (g/dL)	Pre		19.8 ± 0.19	19.77 ± 0.21	19.61 ± 0.24	20.3 ± 0.47
	Post		18.56 ± 0.22	18.28 ± 0.25	17.42 ± 0.21	20.35 ± 0.31
	D21		17.11 ± 1.92	18.14 ± 0.22	18.18 ± 0.30	21.50 ± 0.65
	D42		18.89 ± 0.30	18.03 ± 0.43	18.03 ± 0.33	20.60 ± 0.15
IgG (mg/dL)	Pre		121.9 ± 8.1	167.1 ± 31.5	135.3 ± 10.7	110.9 ± 20.5
IgG (mg/dL)	Post		12.4 ± 11.9*	2.64 ± 2.3*	2.23 ± 0.9*	100.5 ± 15.6
IgM (mg/dL)	Pre		8.3 ± 1.1	8.69 ± 1.2	9.4 ± 1.2	10.0 ± 3.7
IgM (mg/dL)	Post		1.3 ± 0.6*	1.1 ± 0.5*	1.9 ± 0.5*	11.9 ± 3.8
MFI	HLA I	Pre	5568.06 ± 938.78	3037.52 ± 202.22	2682.01 ± 178.84	N/A
	HLA I	Post	<2000	<2000	<2000	N/A
	HLA II	Pre	2837.67 ± 163.46	3163.15 ± 202.09	4339.25 ± 583.68	N/A
	HLA II	Post	<2000	<2000	<2000	N/A
HLA I (# of donors)	Pre		2	2	4†	0
HLA I (# of donors)	Post		0	0	0	0
HLA II (# of donors)	Pre		2	2	5†	0
HLA II (# of donors)	Post		0	0	0	0
2,3-DPG (µmol/gHb)	Pre		253.8 ± 12.4	275.0 ± 12.8	259.6 ± 24.2	276.5 ± 12.7
	Post		221.9 ± 6.0	229.2 ± 8.3	209.6 ± 10.0	248.3 ± 10.2
	D21		28.7 ± 3.8	35.7 ± 5.3	27.7 ± 5.2	25.45 ± 3.5
	D42		14.4 ± 1.0*	13.9 ± 1.6*	13.0 ± 2.3*	12.9 ± 0.9*
ATP (µmol/gHb)	Pre		4.4 ± 0.3	4.8 ± 0.1	4.8 ± 0.1	4.4 ± 0.3
	Post		5.1 ± 0.1	5.2 ± 0.2	5.2 ± 0.1	4.5 ± 0.3
	D21		4.7 ± 0.1	4.5 ± 0.2	4.8 ± 0.1	4.4 ± 0.3
	D42		3.1 ± 0.1*	3.1 ± 0.2*	3.3 ± 0.2*	3.3 ± 0.2
pH range (D42/D0)			6.5/7.3	6.6/7.4	6.4/7.3	6.7/7.3

Hb, hemoglobin; Hct, hematocrit; N/A, not available; Plt, platelet; WBC, white blood cell.

Signifies P < .05 from day (D) 0 preexperimental filtration supernatant (Pre) or standard LR.

†

Indicates that antibodies to both HLA class I and class II were present in the same donor.

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