Distribution of CD20posBCL2posCD10pos triple positive cells in the GC of RLNs
| Sample* . | Triple positive cells/105 cells† . | Triple positive cells in GC . | GC surface (%)‡ . | Enrichment coefficient in GC§ . |
|---|---|---|---|---|
| RLN1 | 39 | 15 (38.5%) | 6.0 | 6.41 |
| RLN2 | 18 | 7 (39%) | 11.3 | 3.45 |
| RLN3 | 15 | 5 (33.3%) | 6.1 | 5.45 |
| RLN4 | 48 | 35 (72.9%) | 11 | 6.63 |
| RLN5 | 53 | 3 (5.7%) | 5.7 | 1 |
| RLN9 | 4 | 0 | 8.3 | 0 |
| RLN10 | 8 | 0 | 4.9 | 0 |
| RLN11 | 5 | 0 | 31.7 | 0 |
| Sample* . | Triple positive cells/105 cells† . | Triple positive cells in GC . | GC surface (%)‡ . | Enrichment coefficient in GC§ . |
|---|---|---|---|---|
| RLN1 | 39 | 15 (38.5%) | 6.0 | 6.41 |
| RLN2 | 18 | 7 (39%) | 11.3 | 3.45 |
| RLN3 | 15 | 5 (33.3%) | 6.1 | 5.45 |
| RLN4 | 48 | 35 (72.9%) | 11 | 6.63 |
| RLN5 | 53 | 3 (5.7%) | 5.7 | 1 |
| RLN9 | 4 | 0 | 8.3 | 0 |
| RLN10 | 8 | 0 | 4.9 | 0 |
| RLN11 | 5 | 0 | 31.7 | 0 |
RLN 1-5 and RLN 9-11 samples correspond to t(14;18)hi and t(14;18)neg LNs, respectively. The frequency of t(14;18) was determined by qPCR on dissociated RLN cells.
Number of CD20posBCL2posCD10pos triple positive cells visualized by immunohistofluorescence. For each sample, between 1 and 3 large (2-5 mm2) representative areas including GC were chosen (corresponding to 7 × 104 to 2 × 105 cells per sample) for counting.
Percentage of the analyzed area occupied by GC zones (defined as areas of CD20posCD10posBcl2neg cells).
Enrichment coefficient = (% triple positive cells in GC)/(% GC surface).