Table 1 List of VWF constructs used in... | American Society of Hematology

Table 1

List of VWF constructs used in this study

Constructs		Purpose
pCMV6-XL5	h VWF/WT	Immunofluorescence/flow assay
	h VWF/p.S1494C
	h VWF/p.S1534C
	h VWF/p.S1494C-p.S1534C
	Vn_SP-A2-His₆	Purification/proteolysis assays
	Vn_SP-A2/p.S1494C-p.S1534C-His₆	Purification/proteolysis assays
pLIVE	mVWF/WT	Hydrodynamic injection in mice
pLIVE	mVWF/p.S1494C-p.A1534C	Hydrodynamic injection in mice

Constructs		Purpose
pCMV6-XL5	h VWF/WT	Immunofluorescence/flow assay
	h VWF/p.S1494C
	h VWF/p.S1534C
	h VWF/p.S1494C-p.S1534C
	Vn_SP-A2-His₆	Purification/proteolysis assays
	Vn_SP-A2/p.S1494C-p.S1534C-His₆	Purification/proteolysis assays
pLIVE	mVWF/WT	Hydrodynamic injection in mice
pLIVE	mVWF/p.S1494C-p.A1534C	Hydrodynamic injection in mice

cDNA encoding either full-length human VWF or its A2 domain was subcloned into pCMV6-XL5, a plasmid that allowed better VWF expression in HEK293T cells than any other vector tested (data not shown). pLIVE constructs were done using the cDNA for mVWF. Of note, mVWF constructs bear a T103M mutation.

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