Transcripts of important immune receptors underrepresented in MCs
| . | MC ex vivo* . | MC cultured† . | Cultured + stimulated‡ . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene . | D2 . | D3 . | D4 . | D1 . | D8 . | D5 . | D1 . | D8 . | D5 . | First primary sample§ . | Mean all|| . |
| Receptors involved in antigen presentation | |||||||||||
| HLA-DRA | 45 | 30 | 25 | 0.1 | 0 | 0 | 0 | 0 | 0.1 | 26 120 (migratory LC) | 798 |
| HLA-DPA1 | 28 | 20 | 16 | 8.8 | 22 | 18 | 10 | 27 | 16 | 13 278 (migratory LC) | 408 |
| HLA-DPB1 | 16 | 13 | 17 | 2 | 4.6 | 7.5 | 0.9 | 13 | 4.2 | 13 319 (migratory LC) | 239 |
| HLA-DQA1 | 0.5 | 1 | 6.2 | 0 | 0.4 | 0 | 0 | 0 | 0 | 17 246 (migratory LC) | 143 |
| HLA-DQA2 | 26 | 1.4 | 4 | 0 | 0 | 0 | 0 | 0 | 0.1 | 3 963 (migratory LC) | 38 |
| Chemokine receptors | |||||||||||
| CCR1 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 | 0 | 705 (stim. monocyte) | 18 |
| CCR2 | 0 | 0.4 | 0.2 | 0 | 1.2 | 0.1 | 0.3 | 0.7 | 0 | 178 (monocyte) | 5.3 |
| CCR3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 190 (whole blood) | 2.3 |
| CCR4 | 2.6 | 0.5 | 1.4 | 21 | 6.6 | 80 | 1.3 | 3.5 | 27 | 1 384 (memory Treg) | 19 |
| CCR5 | 0 | 0 | 0.2 | 0.4 | 2.5 | 0.7 | 0 | 0.7 | 0.8 | 199 (stim. monocyte) | 7.8 |
| CCRL2 | 0 | 0 | 0.2 | 0.4 | 0.2 | 1.2 | 3.4 | 0.7 | 1 | 470 (stim. monocyte) | 8 |
| CXCR3 | 0.7 | 0.4 | 0.5 | 4.2 | 0.2 | 5.6 | 4.1 | 7.7 | 40 | 359 (plasmacytoid DC) | 5.9 |
| IL8RA/CXCR1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 216 (whole blood) | 13 |
| Pattern recognition receptors | |||||||||||
| TLR1 | 0.9 | 1.2 | 1.2 | 0.3 | 1.9 | 0.4 | 0 | 0.7 | 2.4 | 434 (neutrophil) | 12 |
| TLR2 | 4.7 | 3.9 | 2.3 | 0 | 1 | 0 | 0 | 0 | 0.4 | 896 (eosinophil) | 29 |
| TLR3 | 1.4 | 0.7 | 0.5 | 3.9 | 2.5 | 3.4 | 0.3 | 0.7 | 3.6 | 114 (placenta) | 6.5 |
| TLR4 | 3.5 | 11 | 5.4 | 75 | 22 | 44 | 28 | 16 | 28 | 1 085 (stim. monocyte) | 55 |
| TLR7 | 0.9 | 0.4 | 0.8 | 0.1 | 0.2 | 0 | 0 | 0 | 1.5 | 343 (plasmacytoid DC) | 7.7 |
| TLR9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 162 (plasmacytoid DC) | 1.9 |
| . | MC ex vivo* . | MC cultured† . | Cultured + stimulated‡ . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene . | D2 . | D3 . | D4 . | D1 . | D8 . | D5 . | D1 . | D8 . | D5 . | First primary sample§ . | Mean all|| . |
| Receptors involved in antigen presentation | |||||||||||
| HLA-DRA | 45 | 30 | 25 | 0.1 | 0 | 0 | 0 | 0 | 0.1 | 26 120 (migratory LC) | 798 |
| HLA-DPA1 | 28 | 20 | 16 | 8.8 | 22 | 18 | 10 | 27 | 16 | 13 278 (migratory LC) | 408 |
| HLA-DPB1 | 16 | 13 | 17 | 2 | 4.6 | 7.5 | 0.9 | 13 | 4.2 | 13 319 (migratory LC) | 239 |
| HLA-DQA1 | 0.5 | 1 | 6.2 | 0 | 0.4 | 0 | 0 | 0 | 0 | 17 246 (migratory LC) | 143 |
| HLA-DQA2 | 26 | 1.4 | 4 | 0 | 0 | 0 | 0 | 0 | 0.1 | 3 963 (migratory LC) | 38 |
| Chemokine receptors | |||||||||||
| CCR1 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 | 0 | 705 (stim. monocyte) | 18 |
| CCR2 | 0 | 0.4 | 0.2 | 0 | 1.2 | 0.1 | 0.3 | 0.7 | 0 | 178 (monocyte) | 5.3 |
| CCR3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 190 (whole blood) | 2.3 |
| CCR4 | 2.6 | 0.5 | 1.4 | 21 | 6.6 | 80 | 1.3 | 3.5 | 27 | 1 384 (memory Treg) | 19 |
| CCR5 | 0 | 0 | 0.2 | 0.4 | 2.5 | 0.7 | 0 | 0.7 | 0.8 | 199 (stim. monocyte) | 7.8 |
| CCRL2 | 0 | 0 | 0.2 | 0.4 | 0.2 | 1.2 | 3.4 | 0.7 | 1 | 470 (stim. monocyte) | 8 |
| CXCR3 | 0.7 | 0.4 | 0.5 | 4.2 | 0.2 | 5.6 | 4.1 | 7.7 | 40 | 359 (plasmacytoid DC) | 5.9 |
| IL8RA/CXCR1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 216 (whole blood) | 13 |
| Pattern recognition receptors | |||||||||||
| TLR1 | 0.9 | 1.2 | 1.2 | 0.3 | 1.9 | 0.4 | 0 | 0.7 | 2.4 | 434 (neutrophil) | 12 |
| TLR2 | 4.7 | 3.9 | 2.3 | 0 | 1 | 0 | 0 | 0 | 0.4 | 896 (eosinophil) | 29 |
| TLR3 | 1.4 | 0.7 | 0.5 | 3.9 | 2.5 | 3.4 | 0.3 | 0.7 | 3.6 | 114 (placenta) | 6.5 |
| TLR4 | 3.5 | 11 | 5.4 | 75 | 22 | 44 | 28 | 16 | 28 | 1 085 (stim. monocyte) | 55 |
| TLR7 | 0.9 | 0.4 | 0.8 | 0.1 | 0.2 | 0 | 0 | 0 | 1.5 | 343 (plasmacytoid DC) | 7.7 |
| TLR9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 162 (plasmacytoid DC) | 1.9 |
DC, dendritic cell; LC, Langerhans cell; stim., stimulated; Treg, regulatory T cell.
Donor 2/donor 3/donor 4 (directly upon isolation from skin).
Donor 1/donor 8/donor 5 (MCs expanded in culture for 4-5 weeks).
Donor 1 stimulated for 2.5 hours/donor 8 stimulated for 5 hours/donor 5 stimulated for 16 hours.
Nontransformed cell/tissue sample with (second) highest expression of the gene.
Mean expression of all non-MC FANTOM5 samples. The values are given in (rounded) tags per million tags; if <10, 1 decimal place is given.