Expression pattern of genes encoding well-defined MC markers
| Gene . | MC ex vivo* . | MC cultured† . | Cultured + stimulated‡ . | (Next) Primary sample§ . | Mean all|| . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| D2 . | D3 . | D4 . | D1 . | D8 . | D5 . | D1 . | D8 . | D5 . | |||
| KIT | 3 272 | 3 633 | 3268 | 4 426 | 2906 | 3 125 | 2094 | 1 261 | 2195 | 548 (melanocyte) | 16 |
| IL1RL1 | 656 | 1 156 | 418 | 487 | 340 | 423 | 1631 | 488 | 1073 | 423 (lung) | 8 |
| FCER1A | 811 | 657 | 1087 | 2 699 | 1774 | 2 405 | 1211 | 1 670 | 2983 | 3272 (immature LC) | 19 |
| MS4A2 | 1 163 | 1 717 | 1284 | 2 173 | 1109 | 2 827 | 1580 | 533 | 1759 | 60 (basophil) | 1 |
| ENPP3 | 312 | 454 | 273 | 890 | 700 | 920 | 3209 | 1 116 | 1084 | 157 (basophil) | 3 |
| HDC | 994 | 1 091 | 864 | 1 516 | 772 | 925 | 496 | 400 | 707 | 255 (basophil) | 5 |
| TPSAB1-TPSB2 | 2 536 | 1 332 | 2650 | 671 | 250 | 991 | 0 | 57 | 492 | 76 (stimulated MΦ) | 0.1 |
| TPSD1 | 552 | 783 | 558 | 317 | 972 | 1 585 | 40 | 518 | 925 | 5 (gallbladder) | 0.2 |
| CMA1 | 1 841 | 1 919 | 1240 | 631 | 119 | 1 210 | 502 | 203 | 624 | 8 (left atrium) | 0.2 |
| CPA3 | 5 057 | 6 001 | 2824 | 7 089 | 7295 | 7 883 | 7305 | 5 884 | 4386 | 239 (CD34+ diff. ery.) | 4 |
| CTSG | 10 520 | 12 133 | 6902 | 14 981 | 7274 | 16 961 | 7000 | 10 752 | 9271 | 1278 (CD34+ BM) | 37 |
| HPGDS | 1 245 | 1 220 | 1220 | 4 097 | 3088 | 3 545 | 3489 | 2 587 | 3182 | 333 (immature LC) | 5 |
| LTC4S | 199 | 270 | 226 | 1 830 | 3261 | 1 684 | 81 | 1 478 | 329 | 194 (immature LC) | 7 |
| Gene . | MC ex vivo* . | MC cultured† . | Cultured + stimulated‡ . | (Next) Primary sample§ . | Mean all|| . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| D2 . | D3 . | D4 . | D1 . | D8 . | D5 . | D1 . | D8 . | D5 . | |||
| KIT | 3 272 | 3 633 | 3268 | 4 426 | 2906 | 3 125 | 2094 | 1 261 | 2195 | 548 (melanocyte) | 16 |
| IL1RL1 | 656 | 1 156 | 418 | 487 | 340 | 423 | 1631 | 488 | 1073 | 423 (lung) | 8 |
| FCER1A | 811 | 657 | 1087 | 2 699 | 1774 | 2 405 | 1211 | 1 670 | 2983 | 3272 (immature LC) | 19 |
| MS4A2 | 1 163 | 1 717 | 1284 | 2 173 | 1109 | 2 827 | 1580 | 533 | 1759 | 60 (basophil) | 1 |
| ENPP3 | 312 | 454 | 273 | 890 | 700 | 920 | 3209 | 1 116 | 1084 | 157 (basophil) | 3 |
| HDC | 994 | 1 091 | 864 | 1 516 | 772 | 925 | 496 | 400 | 707 | 255 (basophil) | 5 |
| TPSAB1-TPSB2 | 2 536 | 1 332 | 2650 | 671 | 250 | 991 | 0 | 57 | 492 | 76 (stimulated MΦ) | 0.1 |
| TPSD1 | 552 | 783 | 558 | 317 | 972 | 1 585 | 40 | 518 | 925 | 5 (gallbladder) | 0.2 |
| CMA1 | 1 841 | 1 919 | 1240 | 631 | 119 | 1 210 | 502 | 203 | 624 | 8 (left atrium) | 0.2 |
| CPA3 | 5 057 | 6 001 | 2824 | 7 089 | 7295 | 7 883 | 7305 | 5 884 | 4386 | 239 (CD34+ diff. ery.) | 4 |
| CTSG | 10 520 | 12 133 | 6902 | 14 981 | 7274 | 16 961 | 7000 | 10 752 | 9271 | 1278 (CD34+ BM) | 37 |
| HPGDS | 1 245 | 1 220 | 1220 | 4 097 | 3088 | 3 545 | 3489 | 2 587 | 3182 | 333 (immature LC) | 5 |
| LTC4S | 199 | 270 | 226 | 1 830 | 3261 | 1 684 | 81 | 1 478 | 329 | 194 (immature LC) | 7 |
BM, bone marrow; CD34+ diff. ery. = CD34+ cells differentiated along the erythroid pathway; D, donor; LC, Langerhans cell; MΦ, macrophage.
Donor 2/donor 3/donor 4 (directly upon isolation from skin).
Donor 1/donor 8/donor 5 (MCs expanded in culture for 4-5 weeks).
Donor 1 stimulated for 2.5 hours/donor 8 stimulated for 5 hours/donor 5 stimulated for 16 hours.
Nontransformed cell/tissue sample with the (second) highest expression of the gene.
Mean expression of all FANTOM5 non-MC samples. The values are given in (rounded) tags per million tags.